Western Blot (WB)
((1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat MYO5A Polyclonal Antibody | anti-MYO5A antibody
Goat anti-MYO5A Antibody
Western Blot: Approx 200kDa band observed in lysates of cell line Jurkat (calculated MW of 215kDa according to NP_000250.3). Recommended concentration: 1-3ug/ml.
Immunofluorescence: Transverse sections of Mouse Skeletal Muscle shows staining of the fibre membrane and neuromuscular junction. The observed pattern corresponds to earlier findings in literature with different antibodies (Röder et al, Proc Natl Acad Sci U S A.2010 Feb 2;107(5): 2031-6; PMID: 20133847). Recommended concentration: 1-3ug/ml.
Western Blot (WB)
((1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunofluorescence (IF)
((1ug/ml) staining of Mouse Skeletal Muscle (first panel, and in green in third panel). Alpha-bungaratoxin staining in middle panel and in red in third panel. Detected by Fluorescence. Data kindly provided by Dr. Rdiger Rudolf, Karlsruhe, Germany)
Immunofluorescence (IF)
((1ug/ml) staining of Mouse Skeletal Muscle (first panel, and in green in third panel). Alpha-bungaratoxin staining in middle panel and in red in third panel. Detected by Fluorescence. Data kindly provided by Dr. Rdiger Rudolf, Karlsruhe, Germany)
NCBI and Uniprot Product Information
NCBI Description
This gene is one of three myosin V heavy-chain genes, belonging to the myosin gene superfamily. Myosin V is a class of actin-based motor proteins involved in cytoplasmic vesicle transport and anchorage, spindle-pole alignment and mRNA translocation. The protein encoded by this gene is abundant in melanocytes and nerve cells. Mutations in this gene cause Griscelli syndrome type-1 (GS1), Griscelli syndrome type-3 (GS3) and neuroectodermal melanolysosomal disease, or Elejalde disease. Multiple alternatively spliced transcript variants encoding different isoforms have been reported, but the full-length nature of some variants has not been determined. [provided by RefSeq, Dec 2008]
Uniprot Description
MYO5A: Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. May also be required for some polarization process involved in dendrite formation. May be a homodimer, which associates with multiple calmodulin or myosin light chains. Binds MLPH and MYRIP. Interacts with RIPL2, the interaction is required for its role in dendrite formation. Detected in melanocytes. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Motor
Chromosomal Location of Human Ortholog: 15q21
Cellular Component: actin filament; actomyosin; cell soma; cytoplasm; cytosol; early endosome; endoplasmic reticulum; filopodium tip; Golgi apparatus; growth cone; intermediate filament; late endosome; lysosome; melanosome; membrane; myosin complex; neuron projection; peroxisome; photoreceptor outer segment; recycling endosome; ruffle; vesicle
Molecular Function: actin binding; ATP binding; calcium ion binding; calmodulin binding; microfilament motor activity; Rab GTPase binding
Biological Process: actin filament-based movement; cellular response to insulin stimulus; ER localization; exocytosis; insulin secretion; locomotion during locomotory behavior; long-chain fatty acid biosynthetic process; melanin biosynthetic process; melanocyte differentiation; melanosome transport; myelination; odontogenesis; post-Golgi vesicle-mediated transport; regulation of inositol-1,4,5-triphosphate receptor activity; secretory granule localization; synapse organization and biogenesis; synaptic transmission; transport; vesicle transport along actin filament; vesicle-mediated transport; visual perception
Disease: Griscelli Syndrome, Type 1
Research Articles on MYO5A
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Product Notes
The MYO5A myo5a (Catalog #AAA422444) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-MYO5A Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's MYO5A can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunofluorescence (IF). Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: Approx 200kDa band observed in lysates of cell line Jurkat (calculated MW of 215kDa according to NP_000250.3). Recommended concentration: 1-3ug/ml. Immunofluorescence: Transverse sections of Mouse Skeletal Muscle shows staining of the fibre membrane and neuromuscular junction. The observed pattern corresponds to earlier findings in literature with different antibodies (Röder et al, Proc Natl Acad Sci U S A.2010 Feb 2;107(5): 2031-6; PMID: 20133847). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the MYO5A myo5a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ETKQLELDLN. It is sometimes possible for the material contained within the vial of "MYO5A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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