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Western Blot (WB) (Mouse Rnasel Antibody (N-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Mouse Rnasel antibody detected the Mouse Rnasel protein (arrow).)

Rabbit anti-Mouse Rnasel Polyclonal Antibody | anti-Rnasel antibody

Mouse Rnasel Antibody (N-term)

Gene Names
Rnasel; E230029I04Rik
Reactivity
Mouse
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Rnasel; Polyclonal Antibody; Mouse Rnasel Antibody (N-term); 2-5A-dependent ribonuclease; 2-5A-dependent RNase; 3126-; Ribonuclease 4; Ribonuclease L; RNase L; Rns4; anti-Rnasel antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Mouse Rnasel antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140-167 amino acids from the N-terminal region of mouse Rnasel.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
140-167
Sequence Length
735
Applicable Applications for anti-Rnasel antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
MOUSE
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Mouse Rnasel Antibody (N-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Mouse Rnasel antibody detected the Mouse Rnasel protein (arrow).)

Western Blot (WB) (Mouse Rnasel Antibody (N-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Mouse Rnasel antibody detected the Mouse Rnasel protein (arrow).)

Western Blot (WB)

(Mouse Rnasel Antibody (N-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the Rnasel antibody detected the Rnasel protein (arrow).)

Western Blot (WB) (Mouse Rnasel Antibody (N-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the Rnasel antibody detected the Rnasel protein (arrow).)
Related Product Information for anti-Rnasel antibody
Endoribonuclease that functions in the interferon (IFN) antiviral response. In INF treated and virus infected cells, RNASEL probably mediates its antiviral effects through a combination of direct cleavage of single-stranded viral RNAs, inhibition of protein synthesis through the degradation of rRNA, induction of apoptosis, and induction of other antiviral genes. RNASEL mediated apoptosis is the result of a JNK-dependent stress-response pathway leading to cytochrome c release from mitochondria and caspase-dependent apoptosis. Therefore, activation of RNASEL could lead to elimination of virus infected cells under some circumstances. Might play a central role in the regulation of mRNA turnover (By similarity).
Product Categories/Family for anti-Rnasel antibody
References
Ireland, D.D., et al. PLoS Pathog. 5 (10), E1000602 (2009) :
Andersen, J.B., et al. RNA Biol 6(3):305-315(2009)
Salehzada, T., et al. PLoS ONE 4 (10), E7563 (2009) :
Li, X.L., et al. Proc. Natl. Acad. Sci. U.S.A. 105(52):20816-20821(2008)
Malathi, K., et al. Nature 448(7155):816-819(2007)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
83275
NCBI Official Full Name
2-5A-dependent ribonuclease
NCBI Official Synonym Full Names
ribonuclease L (2', 5'-oligoisoadenylate synthetase-dependent)
NCBI Official Symbol
Rnasel
NCBI Official Synonym Symbols
E230029I04Rik
NCBI Protein Information
2-5A-dependent ribonuclease
UniProt Protein Name
2-5A-dependent ribonuclease
UniProt Gene Name
Rnasel
UniProt Synonym Gene Names
Rns4; 2-5A-dependent RNase; RNase L
UniProt Entry Name
RN5A_MOUSE

Uniprot Description

RNASEL: a multifunctional enzyme that possesses protein kinase and endoribonuclease activity, Mediator of interferon action, which play a role in mediating resistance to virus infection and apoptosis. Might play a central role in the regulation of mRNA turnover.

Protein type: Ribonuclease; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); EC 3.1.26.-; Kinase, protein; Other group; Other-Unique family

Cellular Component: nuclear matrix; mitochondrion; cytoplasm

Molecular Function: ribonucleoprotein binding; rRNA binding; nuclease activity; hydrolase activity; RNA binding; endonuclease activity; metal ion binding; transferase activity, transferring phosphorus-containing groups; bidentate ribonuclease III activity; nucleotide binding; ATP binding; ribonuclease activity; protein kinase activity

Biological Process: fat cell differentiation; negative regulation of viral genome replication; positive regulation of transcription from RNA polymerase II promoter; regulation of mRNA stability; mRNA processing; protein amino acid phosphorylation; defense response to virus; rRNA processing

Research Articles on Rnasel

Similar Products

Product Notes

The Rnasel rnasel (Catalog #AAA9207992) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 140-167. The Mouse Rnasel Antibody (N-term) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Rnasel can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the Rnasel rnasel for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rnasel, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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