Rabbit anti-Mouse Rnasel Polyclonal Antibody | anti-RNASEL antibody

Rnasel, NT (Rnasel, Rns4, 2-5A-dependent ribonuclease, Ribonuclease 4, Ribonuclease L)

Cat. Num. AAA6005538

• Gene Names: RNASEL; RNS4; PRCA1
• Reactivity: Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Rnasel; Polyclonal Antibody; NT (Rnasel; Rns4; 2-5A-dependent ribonuclease; Ribonuclease 4; Ribonuclease L); Anti -Rnasel; anti-RNASEL antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-RNASEL antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: Mouse Rnasel antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 139-167 amino acids from the N-terminal region of Mouse Rnasel.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Rnasel Antibody (N-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Mouse Rnasel antibody detected the Mouse Rnasel protein (arrow).)

Western Blot (WB)

(Mouse Rnasel Antibody (N-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the Rnasel antibody detected the Rnasel protein (arrow).)

Related Product Information for anti-RNASEL antibody

Endoribonuclease that functions in the interferon (IFN) antiviral response. In INF treated and virus infected cells, RNASEL probably mediates its antiviral effects through a combination of direct cleavage of single-stranded viral RNAs, inhibition of protein synthesis through the degradation of rRNA, induction of apoptosis, and induction of other antiviral genes. RNASEL mediated apoptosis is the result of a JNK-dependent stress-response pathway leading to cytochrome c release from mitochondria and caspase-dependent apoptosis. Therefore, activation of RNASEL could lead to elimination of virus infected cells under some circumstances. Might play a central role in the regulation of mRNA turnover (By similarity).

Product Categories/Family for anti-RNASEL antibody

Antibodies; Abs to Enzymes; Ribonuclease

NCBI and Uniprot Product Information

• NCBI GI #: 60551728
• NCBI GeneID: 6041
• UniProt Accession #: Q05823; Q5W0L2
• Molecular Weight: 83,533 Da
• NCBI Official Full Name: RNASEL protein
• NCBI Official Synonym Full Names: ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent)
• NCBI Official Symbol: RNASEL
• NCBI Official Synonym Symbols: RNS4; PRCA1
• NCBI Protein Information: 2-5A-dependent ribonuclease; RNase L; ribonuclease 4; 2-5A-dependent RNase; interferon-induced 2-5A-dependent RNase; 2',5'-oligoisoadenylate synthetase-dependent
• UniProt Protein Name: 2-5A-dependent ribonuclease
• Protein Family: 2-5A-dependent ribonuclease
• UniProt Gene Name: RNASEL
• UniProt Synonym Gene Names: RNS4; 2-5A-dependent RNase; RNase L
• UniProt Entry Name: RN5A_HUMAN

NCBI Description

This gene encodes a component of the interferon-regulated 2-5A system that functions in the antiviral and antiproliferative roles of interferons. Mutations in this gene have been associated with predisposition to prostate cancer and this gene is a candidate for the hereditary prostate cancer 1 (HPC1) allele. [provided by RefSeq, Jul 2008]

Uniprot Description

RNASEL: a multifunctional enzyme that possesses protein kinase and endoribonuclease activity, Mediator of interferon action, which play a role in mediating resistance to virus infection and apoptosis. Might play a central role in the regulation of mRNA turnover.

Protein type: Protein kinase, Other; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 3.1.26.-; Ribonuclease; Other group; Other-Unique family

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: nuclear matrix; mitochondrion; cytosol

Molecular Function: endoribonuclease activity; rRNA binding; protein binding; RNA binding; metal ion binding; ATP binding; protein kinase activity

Biological Process: negative regulation of viral genome replication; cytokine and chemokine mediated signaling pathway; positive regulation of transcription from RNA polymerase II promoter; mRNA processing; defense response to virus; protein amino acid phosphorylation; rRNA processing

Disease: Prostate Cancer, Hereditary, 1