Immunocytochemistry (ICC)
(Immunocytochemistry- HES‑4 in HEK293 Human Cell Line.)
Sheep anti-Human HES4 Polyclonal Antibody | anti-HES4 antibody
HES4 (Hairy Family Helix-loop-helix Transcription Factor)
Gene Names
HES4; bHLHb42; HES4
Reactivity
Human
Applications
ELISA, Western Blot, Immunocytochemistry
Purity
Affinity PurifiedPurified by human HES-4 affinity chromatography.
Synonyms
HES4; Polyclonal Antibody; HES4 (Hairy Family Helix-loop-helix Transcription Factor); Anti -HES4 (Hairy Family Helix-loop-helix Transcription Factor); anti-HES4 antibody
Host
Sheep
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Selected for its ability to recognize human HES-4 in the applications listed below. In direct ELISAs and Western Blots, this antibody shows less than 1% crossreactivity with rhHES-1, rhHES-2, rhHES-5, and rhHES-7.
Purity/Purification
Affinity Purified
Purified by human HES-4 affinity chromatography.
Purified by human HES-4 affinity chromatography.
Form/Format
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.
Applicable Applications for anti-HES4 antibody
ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)
Application Notes
Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human HES-4. The detection limit for rhHES-4 is approximately 5 ng/lane under non-reducing and reducing conditions., Immunocytochemistry: This antibody has been used at a concentration of 10ug/ml to detect HES-4 in HEK293 cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4 degree C followed by fluorochrome-coupled anti-sheep IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA., Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human HES-4. The detection limit for rhHES-4 is approximately 0.2 ng/well., Optimal dilutions should be determined by each laboratory for each application.
Immunogen
E. coli-derived rhHES-4 (aa94-221)
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunocytochemistry (ICC)
(Immunocytochemistry- HES‑4 in HEK293 Human Cell Line.)
Immunocytochemistry (ICC)
(Immunocytochemistry- HES‑4 in HEK293 Human Cell Line.)
Related Product Information for anti-HES4 antibody
HES-4 is a 221 aa, 24 kD member of the HES-family of transcriptional repressors. HES-4 contains a species-specific bHLH domain (aa 33-93), a specific orange DNA-binding domain (aa 109-150), a 65 aa Proline-rich SH3-binding domain, and a WRPW Groucho/TLE interacting C- terminal motif. HES-4 is a HES-1 paralog likely to maintain stem cell pluripotency. In the region used as immunogen, the amino acid sequence of human and canine HES-4 are 80% identical.
Product Categories/Family for anti-HES4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
23,523 Da
NCBI Official Full Name
hairy and enhancer of split 4 (Drosophila)
NCBI Official Synonym Full Names
hairy and enhancer of split 4 (Drosophila)
NCBI Official Symbol
HES4
NCBI Official Synonym Symbols
bHLHb42; HES4
NCBI Protein Information
transcription factor HES-4; hHES4; bHLH factor Hes4; OTTHUMP00000221118; class B basic helix-loop-helix protein 42
UniProt Protein Name
Transcription factor HES-4
Protein Family
UniProt Gene Name
HES4
UniProt Synonym Gene Names
BHLHB42
UniProt Entry Name
HES4_HUMAN
Uniprot Description
HES4: Transcriptional repressor. Binds DNA on N-box motifs: 5'-CACNAG-3'.
Chromosomal Location of Human Ortholog: 1p36.33
Cellular Component: nucleus
Molecular Function: protein dimerization activity; DNA binding; transcription factor binding
Biological Process: nervous system development; regulation of transcription, DNA-dependent; transcription, DNA-dependent; cell differentiation
Similar Products
Product Notes
The HES4 hes4 (Catalog #AAA613639) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The HES4 (Hairy Family Helix-loop-helix Transcription Factor) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HES4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC). Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human HES-4. The detection limit for rhHES-4 is approximately 5 ng/lane under non-reducing and reducing conditions., Immunocytochemistry: This antibody has been used at a concentration of 10ug/ml to detect HES-4 in HEK293 cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4 degree C followed by fluorochrome-coupled anti-sheep IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA., Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human HES-4. The detection limit for rhHES-4 is approximately 0.2 ng/well., Optimal dilutions should be determined by each laboratory for each application. Researchers should empirically determine the suitability of the HES4 hes4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HES4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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