Rabbit anti-Human RNA-binding motif protein, Y chromosome, family 1 member A1 Polyclonal Antibody | anti-RBMY1A1 antibody

Rabbit anti-human RNA-binding motif protein, Y chromosome, family 1 member A1 polyclonal Antibody

Cat. Num. AAA1491311

• Gene Names: RBMY1A1; RBM; RBM1; RBM2; RBMY; YRRM1; YRRM2; RBMY1C
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Caprylic Acid Ammonium Sulfate Precipitation Purified
• Synonyms: RNA-binding motif protein; Y chromosome; family 1 member A1; Polyclonal Antibody; Rabbit anti-human RNA-binding motif protein; family 1 member A1 polyclonal Antibody; RNA-binding motif protein 1; RNA-binding motif protein 2; Y chromosome RNA recognition motif 1; hRBMY; RBMY1A1; RBM1; RBM2; YRRM1; YRRM2; anti-RBMY1A1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Caprylic Acid Ammonium Sulfate Precipitation Purified
• Sequence Length: 496

• Applicable Applications for anti-RBMY1A1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugate: Non-conjugated
• Immunogen: Recombinant human RNA-binding motif protein, Y chromosome, family 1 member A1 protein
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: RBMY1A1 antibody at 6ug/mlLane 1: MCF7 whole cell lysateLane 2: 293T whole cell lysateLane 3: Rat gonadal tissueSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 56,52,41 kDaObserved band size: 56 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver tissue using MBS1491311 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human testis tissue using MBS1491311 at dilution of 1:100)

Western Blot (WB)

(Western BlotPositive WB detected in: Rat spleen tissueAll lanes: RBMY1A1 antibody at 2.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 56,52,41 KDaObserved band size: 56 KDa)

Immunofluorescence (IF)

(Immunofluorescent analysis of Hela cells using MBS1491311 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-RBMY1A1 antibody

RNA-binding protein involved in pre-mRNA splicing. Required for sperm development. Acts additively with TRA2B to promote exon 7 inclusion of the survival motor neuron SMN. Binds non-specifically to mRNAs.

NCBI and Uniprot Product Information

• NCBI GI #: 4826974
• NCBI GeneID: 5940
• NCBI Accession #: NP_005049.1
• NCBI GenBank Nucleotide #: NM_005058.2
• UniProt Accession #: P0DJD3; Q15376; Q15377; Q15414; Q6NSB5; Q86VU6; Q8NHR0
• Molecular Weight: 40,651 Da
• NCBI Official Full Name: RNA-binding motif protein, Y chromosome, family 1 member A1
• NCBI Official Synonym Full Names: RNA binding motif protein, Y-linked, family 1, member A1
• NCBI Official Symbol: RBMY1A1
• NCBI Official Synonym Symbols: RBM; RBM1; RBM2; RBMY; YRRM1; YRRM2; RBMY1C
• NCBI Protein Information: RNA-binding motif protein, Y chromosome, family 1 member A1
• UniProt Protein Name: RNA-binding motif protein, Y chromosome, family 1 member A1
• Protein Family: RNA-binding motif protein
• UniProt Gene Name: RBMY1A1
• UniProt Synonym Gene Names: RBM1; RBM2; YRRM1; YRRM2; hRBMY
• UniProt Entry Name: RBY1A_HUMAN

NCBI Description

This gene encodes a protein containing an RNA-binding motif in the N-terminus and four SRGY (serine, arginine, glycine, tyrosine) boxes in the C-terminus. Multiple copies of this gene are found in the AZFb azoospermia factor region of chromosome Y and the encoded protein is thought to be involved in spermatogenesis. Most copies of this locus are pseudogenes, although six highly similar copies have full-length ORFs and are considered functional. Four functional copies of this gene are found within inverted repeat IR2; two functional copies of this gene are found in palindrome P3, along with two copies of PTPN13-like, Y-linked. [provided by RefSeq, Jul 2008]

Uniprot Description

RBMY1A1: RNA-binding protein involved in pre-mRNA splicing. Required for sperm development. Acts additively with TRA2B to promote exon 7 inclusion of the survival motor neuron SMN. Binds non-specifically to mRNAs. Interacts with splicing factor proteins SFRS3/SRP20, TRA2B/SFRS10, KHDRBS1/SAM68 and KHDRBS3.

Protein type: RNA splicing

Chromosomal Location of Human Ortholog: Yq11.223

Cellular Component: nucleus

Molecular Function: mRNA binding; nucleotide binding; protein binding

Biological Process: mRNA processing; regulation of alternative nuclear mRNA splicing, via spliceosome; RNA splicing

Disease: Spermatogenic Failure, Y-linked, 2