Western Blot (WB)
(RN185 Antibody (Center) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the RN185 antibody detected the RN185 protein (arrow).)
Rabbit RN185 Polyclonal Antibody | anti-RN185 antibody
RN185, ID (RNF185, E3 ubiquitin-protein ligase RNF185, RING finger protein 185) (PE)
Reactivity
Human, Hamster, Mouse
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
RN185; Polyclonal Antibody; ID (RNF185; E3 ubiquitin-protein ligase RNF185; RING finger protein 185) (PE); RNF185; RING finger protein 185; anti-RN185 antibody
Host
Rabbit
Reactivity
Human, Hamster, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Sequence Length
192
Applicable Applications for anti-RN185 antibody
Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
RN185 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 91-121 amino acids from the Central region of human RN185.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(RN185 Antibody (Center) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the RN185 antibody detected the RN185 protein (arrow).)
Western Blot (WB)
(RN185 Antibody (Center) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the RN185 antibody detected the RN185 protein (arrow).)
Flow Cytometry (FC/FACS)
(RN185 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(RN185 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB)
(RN185 Antibody (Center) western blot analysis in mouse kidney tissue and K562 cell line lysates (35ug/lane).This demonstrates the RN185 antibody detected the RN185 protein (arrow).)
Western Blot (WB)
(RN185 Antibody (Center) western blot analysis in mouse kidney tissue and K562 cell line lysates (35ug/lane).This demonstrates the RN185 antibody detected the RN185 protein (arrow).)
Product Categories/Family for anti-RN185 antibody
References
Collins, J.E., et al. Genome Biol. 5 (10), R84 (2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
E3 ubiquitin-protein ligase RNF185 isoform 1
NCBI Official Synonym Full Names
ring finger protein 185
NCBI Official Symbol
RNF185
NCBI Protein Information
E3 ubiquitin-protein ligase RNF185
UniProt Protein Name
E3 ubiquitin-protein ligase RNF185
UniProt Gene Name
RNF185
UniProt Entry Name
RN185_HUMAN
Research Articles on RN185
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Product Notes
The RN185 rnf185 (Catalog #AAA6342367) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RN185, ID (RNF185, E3 ubiquitin-protein ligase RNF185, RING finger protein 185) (PE) reacts with Human, Hamster, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RN185 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the RN185 rnf185 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RN185, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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