Western Blot (WB)
(RN185 Antibody (Center) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the RN185 antibody detected the RN185 protein (arrow).)
Rabbit RN185 Polyclonal Antibody | anti-RNF185 antibody
RN185 Antibody (Center)
Western Blot (WB)
(RN185 Antibody (Center) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the RN185 antibody detected the RN185 protein (arrow).)
Western Blot (WB)
(RN185 Antibody (Center) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the RN185 antibody detected the RN185 protein (arrow).)
Flow Cytometry (FC/FACS)
(RN185 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(RN185 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB)
(RN185 Antibody (Center) western blot analysis in mouse kidney tissue and K562 cell line lysates (35ug/lane).This demonstrates the RN185 antibody detected the RN185 protein (arrow).)
Western Blot (WB)
(RN185 Antibody (Center) western blot analysis in mouse kidney tissue and K562 cell line lysates (35ug/lane).This demonstrates the RN185 antibody detected the RN185 protein (arrow).)
NCBI and Uniprot Product Information
Uniprot Description
RNF185: Mitochondrial E3 ubiquitin-protein ligase that regulates selective mitochondrial autophagy by mediating 'Lys-63'-linked polyubiquitination of BNIP1. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 6.3.2.-; Membrane protein, multi-pass; Membrane protein, integral; Ligase; Ubiquitin ligase; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 22q12.2
Cellular Component: mitochondrial outer membrane; endoplasmic reticulum membrane; integral to membrane
Molecular Function: zinc ion binding; ligase activity
Biological Process: protein ubiquitination; autophagy
Research Articles on RNF185
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Product Notes
The RNF185 rnf185 (Catalog #AAA9210842) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 88-117. The RN185 Antibody (Center) reacts with Human, hamster, mouse (Predicted Reactivity: Chicken, Zebrafish, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's RN185 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the RNF185 rnf185 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RN185, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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