Rabbit anti-Human EFP Polyclonal Antibody | anti-EFP antibody

EFP (D222) Polyclonal Antibody

Cat. Num. AAA3002125

• Gene Names: TRIM25; EFP; Z147; RNF147; ZNF147
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: EFP; Polyclonal Antibody; EFP (D222) Polyclonal Antibody; E3 ubiquitin/ISG15 ligase TRIM25; Estrogen-responsive finger protein; RING finger protein 147; Tripartite motif-containing protein 25; Ubiquitin/ISG15-conjugating enzyme TRIM25; Zinc finger protein 147; TRIM25; RNF147; ZNF147; anti-EFP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: EFP (D222) polyclonal antibody detects endogenous levels of EFP protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 630

• Applicable Applications for anti-EFP antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 360-418 of Human EFP.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of EFP (D222)  pAb at 1:1000 dilutionLane1:H1792 whole cell lysate (10ug) Lane2:HepG2 whole cell lysate (10ug) Lane3:MCF-7 whole cell lysate (10ug) Lane4:L02 whole cell lysate (10ug))

Western Blot (WB)

(Western Blot (WB) analysis of EFP (D222) pAb at 1:500 dilutionLane1:HEK293T whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug))

Related Product Information for anti-EFP antibody

EFP (estrogen-responsive finger protein) is a transcription factor, the content of which is regulated by estrogen. It has been identified as a member of the RING finger family, a family of proteins containing a Zn2+ binding domain designated the C3HC4 or RING finger. EFP also contains two B box domains and a coiled-coil region (a transactivation domain), which are characteristic of a subgroup of the RING finger family. Estrogen regulates the growth, differentiation and function of target cells in a variety of tissues; however, few genes have been shown to be directly regulated by estrogen. It has been speculated that EFP may mediate estrogen activity in a signaling cascade in which estrogen-ER binding to the estrogen responsive element (ERE) downstream of the EFP gene upregulates EFP gene expression. The EFP gene product may then activate transcription of secondary estrogen responsive genes. Additional studies indicate that the EFP promoter may be regulated by multiple elements and their interacting factors.

NCBI and Uniprot Product Information

• NCBI GI #: 68160937
• NCBI GeneID: 7706
• NCBI Accession #: NP_005073.2
• NCBI GenBank Nucleotide #: NP_005073.2
• UniProt Accession #: Q14258
• Molecular Weight: ~ 71kDa
• NCBI Official Full Name: E3 ubiquitin/ISG15 ligase TRIM25
• NCBI Official Synonym Full Names: tripartite motif containing 25
• NCBI Official Symbol: TRIM25
• NCBI Official Synonym Symbols: EFP; Z147; RNF147; ZNF147
• NCBI Protein Information: E3 ubiquitin/ISG15 ligase TRIM25
• UniProt Protein Name: E3 ubiquitin/ISG15 ligase TRIM25
• Protein Family: Elongation factor
• UniProt Gene Name: TRIM25
• UniProt Synonym Gene Names: EFP; RNF147; ZNF147
• UniProt Entry Name: TRI25_HUMAN

NCBI Description

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TRIM25: a ubiquitous protein that mediates estrogen action in various target organs. Contains one RING-type zinc finger and one SPRY domain.

Protein type: EC 6.3.2.n3; Ubiquitin conjugating system; Ubiquitin ligase; Nuclear receptor co-regulator; Transcription factor; Ligase; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 17q23.2

Cellular Component: nucleoplasm; cytoplasm; cytosol

Molecular Function: protein binding; acid-amino acid ligase activity; zinc ion binding; transcription factor activity

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; negative regulation of virion penetration into host cell; cytokine and chemokine mediated signaling pathway; protein ubiquitination; regulation of virion penetration into host cell; activation of NF-kappaB transcription factor; response to vitamin D; response to estrogen stimulus; innate immune response; positive regulation of transcription factor activity; negative regulation of interferon type I production; defense response to virus