Western Blot (WB)
(Figure A. Representative image of western blot with ACE2 and TMPRSS2 antibody.1) Cell lysate from HEK293-ACE2-TMPRSS2 cells ("ACE2-TMPRSS2", Catalog # MBS434319);2) Cell lysate from HEK293-ACE2 cells ("ACE2").)
Transmembrane Protease, Serine 2 (TMPRSS-2) Cell Line | TMPRSS-2 cell line
Human Transmembrane Protease, Serine 2 (TMPRSS-2) Stable Cell Line
Synonyms
Transmembrane Protease; Serine 2 (TMPRSS-2); Human Transmembrane Protease; Serine 2 (TMPRSS-2) Stable Cell Line; TMPRSS-2 cell line
Form/Format
Stable ACE2-TMPRSS2 cells: 1 mL (2 x 10^6 cells/mL in DMEM, 10% FBS, 10% DMSO)
Application Notes
Cell based high-throughput screening of human TMPRSS2 antagonists
SARS-CoV-2 entry study
SARS-CoV-2 entry study
Characterizations
High level expression;
Culture medium: 90%DMEM+10%FBS+1 ug/ml puromycin +250 ug/ml G418
Culture medium: 90%DMEM+10%FBS+1 ug/ml puromycin +250 ug/ml G418
Parental Cells
HEK293-ACE2 cells
Functional Tests
This cell line has been verified by a TMPRSS2 specific antibody and an ACE2 specific antibody staining.
Survival rate: more than 2.5 million/vial on the second day after thawing
Survival rate: more than 2.5 million/vial on the second day after thawing
Mycoplasma Contamination Test
This lot of cells have been tested and found to be free of mycoplasma contamination.
Cell Culture Medium
Growth medium: 90%DMEM+10%FBS+1 ug/ml puromycin +250 ug/ml G418
Freezing medium: 10% DMSO, 90% complete cell culture medium
Freezing medium: 10% DMSO, 90% complete cell culture medium
Preparation and Storage
Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130 degree C, preferably in liquid nitrogen vapor, until ready for use.
Western Blot (WB)
(Figure A. Representative image of western blot with ACE2 and TMPRSS2 antibody.1) Cell lysate from HEK293-ACE2-TMPRSS2 cells ("ACE2-TMPRSS2", Catalog # MBS434319);2) Cell lysate from HEK293-ACE2 cells ("ACE2").)
Western Blot (WB)
(Figure A. Representative image of western blot with ACE2 and TMPRSS2 antibody.1) Cell lysate from HEK293-ACE2-TMPRSS2 cells ("ACE2-TMPRSS2", Catalog # MBS434319);2) Cell lysate from HEK293-ACE2 cells ("ACE2").)
Data
(Figure B. SARS-CoV-2 Omicron Pseudoviral Particle “Infection” of ACE2 and ACE2-TMPRSS2 expressed cells.7.5K of HEK293-ACE2 or HEK293-ACE2-TMPRSS2 cells were plated to a 384-well white clear plate coated with PDL. On the 2nd day, the cells were infected with 12.5 ml of SARS-CoV2-Omicron Pseudovirus Particles ("Omicron-PP", Catalog # MBS434315) and cultured for additional 42 hrs. The cells were lysed and the firefly luciferase activity was measured with luciferase assay kit (MBS434279). The reading was normalized by the cell number measured with cell growth assay kit (CA-A115).)
Data
(Figure B. SARS-CoV-2 Omicron Pseudoviral Particle “Infection” of ACE2 and ACE2-TMPRSS2 expressed cells.7.5K of HEK293-ACE2 or HEK293-ACE2-TMPRSS2 cells were plated to a 384-well white clear plate coated with PDL. On the 2nd day, the cells were infected with 12.5 ml of SARS-CoV2-Omicron Pseudovirus Particles ("Omicron-PP", Catalog # MBS434315) and cultured for additional 42 hrs. The cells were lysed and the firefly luciferase activity was measured with luciferase assay kit (MBS434279). The reading was normalized by the cell number measured with cell growth assay kit (CA-A115).)
Related Product Information for TMPRSS-2 cell line
This HEK293-hTMPRSS2-hACE2 stable cell line expresses a recombinant human Transmembrane Protease, Serine 2 (TMPRSS-2) and a human Angiotensin-Converting Enzyme 2 (ACE2). It can be used for drug screening and for studying SARS-CoV-2 viral entry.
The TMPRSS2 gene encodes a protein that belongs to the serine protease family. The encoded protein, Transmembrane Protease, Serine 2, contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. This protein also facilitates entry of viruses into host cells by proteolytically cleaving and activating viral envelope glycoproteins. Viruses found to use this protein for cell entry include Influenza virus and the human coronaviruses HCoV-229E, MERS-CoV, SARS-CoV and SARS-CoV-2 (COVID-19 virus). The ACE2, Angiotensin-Converting Enzyme 2, is the main receptor protein for SARS-CoV-2 Spike protein
The TMPRSS2 gene encodes a protein that belongs to the serine protease family. The encoded protein, Transmembrane Protease, Serine 2, contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. This protein also facilitates entry of viruses into host cells by proteolytically cleaving and activating viral envelope glycoproteins. Viruses found to use this protein for cell entry include Influenza virus and the human coronaviruses HCoV-229E, MERS-CoV, SARS-CoV and SARS-CoV-2 (COVID-19 virus). The ACE2, Angiotensin-Converting Enzyme 2, is the main receptor protein for SARS-CoV-2 Spike protein
NCBI and Uniprot Product Information
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Product Notes
The TMPRSS-2 (Catalog #AAA434319) is a Cell Line and is intended for research purposes only. The product is available for immediate purchase. Cell based high-throughput screening of human TMPRSS2 antagonists SARS-CoV-2 entry study. Researchers should empirically determine the suitability of the TMPRSS-2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Transmembrane Protease, Serine 2 (TMPRSS-2), Cell Line" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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