Rabbit anti-Mouse, Rat RIP3 Polyclonal Antibody | anti-RIP3 antibody

RIP3 Antibody

Cat. Num. AAA840844

• Gene Names: Ripk3; Rip3; AW107945; 2610528K09Rik
• Reactivity: Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: RIP3; Polyclonal Antibody; RIP3 Antibody; RIP like protein kinase 3; RIPK3; anti-RIP3 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.5 mg/ml) immunoaffinity purified rabbit anti-RIP3 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.; Appearance: Colorless liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 422

• Applicable Applications for anti-RIP3 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting (0.5-4 ug/ml) and in Immunohistochemistry (10-20 ug/ml). Rat kidney tissue section can be used as a positive control. An approximately 57 kDa band should be detected.
• Immunogen: Synthetic peptide corresponding to residues surrounding amino acid 476 of mouse RIP3
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-RIP3 antibody

Background: Receptor interacting protein including RIP and RIP2/RICK mediate apoptosis induced by TNFR1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3. RIP3 contains N-terminal kinase domain but, unlike RIP or RIP2, lacks the c-terminal death or CARD domain. RIP3 binds to RIP and TNFR1, and mediates TNF-R1 mediated apoptosis, and attenuates RIP and TNFR1 induced NFkB activation. Overexpression of RIP3 induces apoptosis and NFkB activation.

Product Categories/Family for anti-RIP3 antibody

Signal Transduction; Protein Kinase; Kinase AntibodiesAntibodies & Supporting Tools; Primary Antibodies (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 256017161
• NCBI GeneID: 56532
• NCBI Accession #: NP_001157580
• NCBI GenBank Nucleotide #: NM_001164108.1
• Molecular Weight: 53,322 Da
• NCBI Official Full Name: receptor-interacting serine/threonine-protein kinase 3 isoform 2
• NCBI Official Synonym Full Names: receptor-interacting serine-threonine kinase 3
• NCBI Official Symbol: Ripk3
• NCBI Official Synonym Symbols: Rip3; AW107945; 2610528K09Rik
• NCBI Protein Information: receptor-interacting serine/threonine-protein kinase 3
• UniProt Protein Name: Receptor-interacting serine/threonine-protein kinase 3
• Protein Family: Putative zinc metalloprotease
• UniProt Gene Name: Ripk3
• UniProt Synonym Gene Names: Rip3; RIP-3; mRIP3
• UniProt Entry Name: RIPK3_MOUSE

Uniprot Description

RIPK3: Promotes apoptosis. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Binds TRAF2 and RIPK1 and is recruited to the TNFR-1 signaling complex. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); TKL group; RIPK family

Cellular Component: membrane; mitochondrion; cytoplasm; plasma membrane; intracellular; cytosol

Molecular Function: NF-kappaB-inducing kinase activity; transferase activity; protein serine/threonine kinase activity; identical protein binding; protein binding; transferase activity, transferring phosphorus-containing groups; protein complex binding; nucleotide binding; kinase activity; ATP binding; protein kinase activity

Biological Process: spleen development; regulation of activated T cell proliferation; I-kappaB kinase/NF-kappaB cascade; viral reproduction; thymus development; protein heterooligomerization; protein amino acid autophosphorylation; programmed cell death; T cell differentiation in the thymus; positive regulation of transferase activity; protein amino acid phosphorylation; lymph node development; activation of NF-kappaB transcription factor; regulation of T cell mediated cytotoxicity; regulation of adaptive immune response; regulation of interferon-gamma production; activation of protein kinase activity; T cell homeostasis; positive regulation of ligase activity; phosphorylation; protein homooligomerization; positive regulation of oxidoreductase activity