Rabbit NHERF1/EBP50 Polyclonal Antibody | anti-NHERF1/EBP50 antibody

NHERF1 / EBP50 Antibody

Cat. Num. AAA840625

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: NHERF1/EBP50; Polyclonal Antibody; NHERF1 / EBP50 Antibody; Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Ezrin-radixin-moesin-binding phosphoprotein 50; Regulatory cofactor of Na(+)/H(+) exchanger; Sodium-hydrogen exchanger regulatory factor 1; Solute carrier family 9 isoform A3 regulatory factor 1; anti-NHERF1/EBP50 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.5 mg/ml) affinity purified rabbit anti- NHERF1polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.; Appearance: Colorless liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 356

• Applicable Applications for anti-NHERF1/EBP50 antibody: Western Blot (WB)
• Application Notes: Western Blot analysis (1-4 ug/ml).
• Immunogen: Synthetic peptide surrounding amino acid 338of rat NHERF1
• Positive Control: Rat kidney tissue lysate
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-NHERF1/EBP50 antibody

Background: NHE3 regulatory factor 1 (NHERF-1) is a multifunctional adaptor protein that interacts with NHE3 via its PDZ domains. The amino-terminal PDZ domain regulates the dimerization of NHERF-1 in vivo. G protein-coupled receptor kinase 6A phosphorylates NHERF-1 at Ser 289 via a PDZ domain-mediated interaction.

Product Categories/Family for anti-NHERF1/EBP50 antibody

Signal Transduction; Protein Kinases; Kinase AntibodiesAntibodies & Supporting Tools; Primary Antibodies (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 11024674
• NCBI GeneID: 59114
• NCBI Accession #: NP_067605.1
• NCBI GenBank Nucleotide #: NM_021594.1
• UniProt Accession #: Q9JJ19
• Molecular Weight: 38,830 Da
• NCBI Official Full Name: Na(+)/H(+) exchange regulatory cofactor NHE-RF1
• NCBI Official Synonym Full Names: solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1
• NCBI Official Symbol: Slc9a3r1
• NCBI Protein Information: Na(+)/H(+) exchange regulatory cofactor NHE-RF1
• UniProt Protein Name: Na(+)/H(+) exchange regulatory cofactor NHE-RF1
• UniProt Gene Name: Slc9a3r1
• UniProt Synonym Gene Names: Nherf; Nherf1; NHERF-1; EBP50
• UniProt Entry Name: NHRF1_RAT

NCBI Description

a membrane-cytoskeleton linking protein thought to be involved in sequestering and regulating the activity of integral membrane proteins, and in modulating membrane protein trafficking [RGD, Feb 2006]

Uniprot Description

NHERF: a scaffold protein that connects plasma membrane proteins with members of the Ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized beta-2-adrenergic receptor Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Cellular Component: centrosome; microvillus; intracellular membrane-bound organelle; brush border membrane; cell; endomembrane system; stereocilium bundle tip; lipid raft; ruffle; stereocilium; membrane; apical part of cell; microvillus membrane; cytoplasm; apical plasma membrane; plasma membrane; intracellular; nucleus; vesicle; filopodium

Molecular Function: protein domain specific binding; protein binding; chloride channel regulator activity; protein self-association; beta-2 adrenergic receptor binding; protein complex binding; beta-catenin binding; dopamine receptor binding; protein complex scaffold; receptor binding; phosphatase binding; PDZ domain binding

Biological Process: bile acid secretion; Wnt receptor signaling pathway; negative regulation of protein kinase B signaling cascade; renal sodium ion transport; negative regulation of cell proliferation; microvillus biogenesis; cAMP-mediated signaling; cellular phosphate ion homeostasis; sensory perception of sound; auditory receptor cell stereocilium organization and biogenesis; dopamine receptor, phospholipase C activating pathway; negative regulation of sodium:hydrogen antiporter activity; regulation of protein kinase activity; actin cytoskeleton organization and biogenesis; negative regulation of cell migration; dopamine receptor, adenylate cyclase activating pathway; negative regulation of phosphoinositide 3-kinase cascade