Mouse anti-Human RIP3 Monoclonal Antibody | anti-RIPK3 antibody

RIP3 (Receptor Interacting Protein 3)

Cat. Num. AAA603758

• Gene Names: RIPK3; RIP3
• Reactivity: Human
• Applications: Western Blot
• Purity: Highly Purified; Highly purified (SDS-PAGE).
• Synonyms: RIP3; Monoclonal Antibody; RIP3 (Receptor Interacting Protein 3); Anti -RIP3 (Receptor Interacting Protein 3); anti-RIPK3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 6D746
• Specificity: Recognizes human RIP3.
• Purity/Purification: Highly Purified; Highly purified (SDS-PAGE).
• Form/Format: Liquid. Purified antibody in PBS containing 0.02% sodium azide.

• Applicable Applications for anti-RIPK3 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Immunogen: Recombinant human RIP3.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Figure: Detection of human RIP3 Method: HEK 293T cells (5x105) mock transfected (lane 1) or transfected (lane 2) with an expression plasmid coding for human RIP3 were harvested, lysed and cell extracts (5x105) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the monoclonal antibody at a 1:1000 dilution for 2 hours. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG and a chemiluminescence detection system.)

Related Product Information for anti-RIPK3 antibody

Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNF-R1 and Fas, two prototype members in the death receptor family. A member in the RIP kinase family was identified and designated RIP3 [1-3]. RIP3 contains an N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNF-R1, mediates TNF-R1 induced apoptosis, and attenuates RIP and TNF-R1 induced NF- B activation. Overexpression of RIP3 induces apoptosis and NF- B activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues [2,3]. Meanwhile RIP4 has been identified and initially characterized. These data suggest that RIP4 is involved in NF- B and JNK signalling and that caspase-dependent processing of RIP4 may negatively regulate NF- B-dependent pro-survival or pro-inflammatory signals [4].

Product Categories/Family for anti-RIPK3 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 40254844
• NCBI GeneID: 11035
• NCBI Accession #: NP_006862.2
• NCBI GenBank Nucleotide #: NM_006871.3
• UniProt Accession #: Q9Y572; Q5J795; Q5J796; Q6P5Y1; B4DJL9; C4AM87
• Molecular Weight: 56,887 Da
• NCBI Official Full Name: receptor-interacting serine/threonine-protein kinase 3
• NCBI Official Synonym Full Names: receptor-interacting serine-threonine kinase 3
• NCBI Official Symbol: RIPK3
• NCBI Official Synonym Symbols: RIP3
• NCBI Protein Information: receptor-interacting serine/threonine-protein kinase 3; RIP-3; RIP-like protein kinase 3; receptor interacting protein 3; receptor-interacting protein 3
• UniProt Protein Name: Receptor-interacting serine/threonine-protein kinase 3
• Protein Family: Receptor-interacting serine/threonine-protein kinase
• UniProt Gene Name: RIPK3
• UniProt Synonym Gene Names: RIP3; RIP-3
• UniProt Entry Name: RIPK3_HUMAN

NCBI Description

The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor. [provided by RefSeq, Jul 2008]

Uniprot Description

RIPK3: Promotes apoptosis. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Binds TRAF2 and RIPK1 and is recruited to the TNFR-1 signaling complex. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.11.1; Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; TKL group; RIPK family

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: mitochondrion; plasma membrane; cytosol

Molecular Function: NF-kappaB-inducing kinase activity; identical protein binding; protein serine/threonine kinase activity; protein binding; transcription coactivator activity; protein complex binding; ATP binding; protein kinase activity

Biological Process: spleen development; regulation of activated T cell proliferation; I-kappaB kinase/NF-kappaB cascade; thymus development; protein heterooligomerization; protein amino acid autophosphorylation; MyD88-independent toll-like receptor signaling pathway; T cell differentiation in the thymus; protein modification process; toll-like receptor 3 signaling pathway; signal transduction; lymph node development; activation of NF-kappaB transcription factor; regulation of T cell mediated cytotoxicity; regulation of adaptive immune response; regulation of interferon-gamma production; toll-like receptor signaling pathway; activation of protein kinase activity; positive regulation of interferon type I production; innate immune response; T cell homeostasis; positive regulation of ligase activity; toll-like receptor 4 signaling pathway; protein homooligomerization; positive regulation of oxidoreductase activity