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Immunofluorescence (IF) (Immunofluorescence staining of Hela cells with CSB-PA882116LA01HU at 1:333, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)

Rabbit anti-Human DDX18 Polyclonal Antibody | anti-DDX18 antibody

DDX18 Antibody

Gene Names
DDX18; Has1; MrDb
Reactivity
Human
Applications
ELISA, Immunofluorescence
Purity
>95%
Protein G Purified
Synonyms
DDX18; Polyclonal Antibody; DDX18 Antibody; ATP-dependent RNA helicase DDX18; EC 3.6.4.13; DEAD box protein 18; Myc-regulated DEAD box protein; MrDb; cPERP-D; anti-DDX18 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%
Protein G Purified
Form/Format
Liquid
Sequence Length
670
Applicable Applications for anti-DDX18 antibody
ELISA (EIA), Immunofluorescence (IF)
Application Notes
IF: 1:200-1:500
Immunogen Species
Human
Immunogen
Recombinant Human ATP-dependent RNA helicase DDX18 protein (32-110AA)
Conjugate
Non-Conjugated
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at-20 degree C or-80 degree C. Avoid repeated freeze.

Immunofluorescence (IF)

(Immunofluorescence staining of Hela cells with CSB-PA882116LA01HU at 1:333, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)

Immunofluorescence (IF) (Immunofluorescence staining of Hela cells with CSB-PA882116LA01HU at 1:333, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
Product Categories/Family for anti-DDX18 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
75,407 Da
NCBI Official Full Name
ATP-dependent RNA helicase DDX18
NCBI Official Synonym Full Names
DEAD-box helicase 18
NCBI Official Symbol
DDX18
NCBI Official Synonym Symbols
Has1; MrDb
NCBI Protein Information
ATP-dependent RNA helicase DDX18
UniProt Protein Name
ATP-dependent RNA helicase DDX18
UniProt Gene Name
DDX18
UniProt Synonym Gene Names
; MrDb

NCBI Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, and it is activated by Myc protein. [provided by RefSeq, Jul 2008]

Uniprot Description

Probable RNA-dependent helicase.

Research Articles on DDX18

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Product Notes

The DDX18 ddx18 (Catalog #AAA7114606) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DDX18 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DDX18 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF). IF: 1:200-1:500. Researchers should empirically determine the suitability of the DDX18 ddx18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DDX18, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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