Human Osteoadherin ELISA Kit | OMD elisa kit

Human Osteoadherin PicoKine ELISA Kit

Cat. Num. AAA1752624

• Gene Names: OMD; OSAD; SLRR2C
• Reactivity: Human
• Synonyms: Osteoadherin; Human Osteoadherin PicoKine ELISA Kit; Human Osteoadherin; Osteomodulin; Keratan sulfate proteoglycan osteomodulin; KSPG osteomodulin; OSAD; OMD; SLRR2C; UNQ190; PRO216; OMD elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human OMD
• Sequence Length: 421

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for OMD elisa kit

Principle of the Assay: The Picokine™ Human OMD Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human OMD with a 96-well strip plate that is pre-coated with antibody specific for OMD. The detection antibody is a biotinylated antibody specific for OMD. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human OMD with immunogen: Expression system for standard: NSO, Immunogen sequence: Q21-E421. The kit is analytically validated with ready to use reagents. To measure Human OMD, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human OMD in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human OMD in the sample.!!Background/Introduction: Osteomodulin (also called osteoadherin or osteoadherin proteoglycan) is a protein that in humans is encoded by the OMD gene. This gene is mapped to 9q22.31. Osteoadherin (OSAD), also known as Osteomodulin, is an extracellular matrix keratan sulfate proteoglycan that belongs to the class II subfamily of small leucine-rich proteoglycans (SLRP). LRR motifs consist of approximately amino acids (aa) with conserved leucine spacing, folded into a structure with one beta -sheet and one alpha -helix. Human OSAD is expressed as an even larger 110 kDa molecule in teeth. OSAD contains eight sulfated tyrosine residues and is distinguished from other class II SLRPs by the presence of an approximately terminal acidic domain. OSAD is expressed by fetal and adult osteoblasts but is not detectable in cartilage or tendon. In dental tissue, OSAD is expressed by odontoblasts and ameloblasts and is involved in the mineralization of bone and teeth. OSAD promotes the adhesion of osteoblasts and odontoblasts to the surrounding matrix, an interaction that is mediated by Integrin alpha V beta 3.

NCBI and Uniprot Product Information

• NCBI GI #: 4826876
• NCBI GeneID: 4958
• NCBI Accession #: NP_005005
• NCBI GenBank Nucleotide #: NP_005005.1
• UniProt Accession #: Q99983
• NCBI Official Full Name: osteomodulin
• NCBI Official Synonym Full Names: osteomodulin
• NCBI Official Symbol: OMD
• NCBI Official Synonym Symbols: OSAD; SLRR2C
• NCBI Protein Information: osteomodulin
• UniProt Protein Name: Osteomodulin
• Protein Family: Osteomodulin
• UniProt Gene Name: OMD
• UniProt Synonym Gene Names: SLRR2C; KSPG osteomodulin; OSAD
• UniProt Entry Name: OMD_HUMAN