Rabbit anti-Human RPL8 Polyclonal Antibody | anti-RPL8 antibody

RPL8, CT (RPL8, 60S ribosomal protein L8) (AP)

Cat. Num. AAA6343589

• Gene Names: RPL8; L8
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RPL8; Polyclonal Antibody; CT (RPL8; 60S ribosomal protein L8) (AP); 60S ribosomal protein L8; anti-RPL8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-RPL8 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RPL8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 206-236 amino acids from the C-terminal region of human RPL8.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(RPL8 Antibody (C-term) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the RPL8 antibody detected the RPL8 protein (arrow).)

Related Product Information for anti-RPL8 antibody

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L2P family of ribosomal proteins. It is located in the cytoplasm. In rat, the protein associates with the 5.8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface. Alternatively spliced transcript variants encoding the same protein exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq].

Product Categories/Family for anti-RPL8 antibody

Antibodies; Ribosomal Proteins

References

Salas, S., et al. Int. J. Cancer 125(4):851-860(2009). Stelzl, U., et al. Cell 122(6):957-968(2005). Andersen, J.S., et al. Nature 433(7021):77-83(2005). Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005). Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 15431306
• NCBI GeneID: 6132
• NCBI Accession #: NP_150644.1; NP_000964.1
• NCBI GenBank Nucleotide #: NM_033301.1
• UniProt Accession #: P62917; P25120; Q567Q7; Q969V7; Q9BWQ9; A8K094; D3DWN2
• Molecular Weight: 28,025 Da
• NCBI Official Full Name: 60S ribosomal protein L8
• NCBI Official Synonym Full Names: ribosomal protein L8
• NCBI Official Symbol: RPL8
• NCBI Official Synonym Symbols: L8
• NCBI Protein Information: 60S ribosomal protein L8
• UniProt Protein Name: 60S ribosomal protein L8
• Protein Family: 60S ribosomal protein
• UniProt Gene Name: RPL8
• UniProt Entry Name: RL8_HUMAN

Uniprot Description

RPL8: a ribosomal protein of the L2P family. Located in the cytoplasm. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This protein is a component of the 60S subunit. Associates with 5.8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface.

Protein type: Ribosomal; Translation; RNA-binding

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: focal adhesion; membrane; cytoplasm; nucleolus; cytosol

Molecular Function: rRNA binding; structural constituent of ribosome; RNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; translational elongation; cellular protein metabolic process; viral reproduction; translation; translational initiation; mRNA catabolic process, nonsense-mediated decay; gene expression; viral transcription; viral infectious cycle; translational termination