Western Blot (WB)
(Sample Data: Western blot analysis of RHEB in mouse brain cell lysate with RHEB antibody at (A) 1, (B) 2, and (C) 4 ug/ml.)
Rabbit RHEB Polyclonal Antibody | anti-RHEB antibody
Anti-RHEB
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA, Immunofluorescence, Immunohistochemistry
Purity
Affinity chromatography
Synonyms
RHEB; Polyclonal Antibody; Anti-RHEB; anti-RHEB antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the RHEB protein
Purity/Purification
Affinity chromatography
Form/Format
PBS + 0.02% sodium azide
Applicable Applications for anti-RHEB antibody
Western Blot (WB), ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC)
Immunogen
RHEB antibody was raised against a 15 amino acid synthetic peptide from the middle region of human RHEB
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
1 year at -20 degree C from date of shipment
1 year at -20 degree C from date of shipment
Western Blot (WB)
(Sample Data: Western blot analysis of RHEB in mouse brain cell lysate with RHEB antibody at (A) 1, (B) 2, and (C) 4 ug/ml.)
Western Blot (WB)
(Sample Data: Western blot analysis of RHEB in mouse brain cell lysate with RHEB antibody at (A) 1, (B) 2, and (C) 4 ug/ml.)
Immunohistochemistry (IHC)
(Sample Data: Immunohistochemistry of RHEB in mouse brain tissue with RHEB antibody at 2 ug/ml.)
Immunohistochemistry (IHC)
(Sample Data: Immunohistochemistry of RHEB in mouse brain tissue with RHEB antibody at 2 ug/ml.)
Related Product Information for anti-RHEB antibody
RHEB is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. RHEB is vital in the regulation of growth and cell cycle progression. RHEB has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form. Drosophila RHEB is a direct target of Tsc2 GTPase-activating protein (GAP) activity both in vivo and in vitro. Point mutations in the Drosophila RHEB gene inhibits cell growth while overexpression promotes cell growth. RHEB functions downstream of Tsc1 and Tsc2 in the mTOR signaling pathway.
Product Categories/Family for anti-RHEB antibody
References
1. Stocker, H. et al: Rheb is an essential regulator of S6K in controlling cell growth in Drosophila. Nature Cell Biol. 5: 559-565, 2003.
2. Zhang, Y. et al: Rheb is a direct target of the tuberous sclerosis tumour suppressor proteins. Nature Cell Biol. 5: 578-581, 2003.
2. Zhang, Y. et al: Rheb is a direct target of the tuberous sclerosis tumour suppressor proteins. Nature Cell Biol. 5: 578-581, 2003.
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Product Notes
The RHEB (Catalog #AAA516197) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RHEB reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RHEB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the RHEB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RHEB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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