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Western Blot (WB) (Western blot analysis of extracts from Mouse liver tissue?, using RGN/SMP30 Antibody. The lane on the left was treated with blocking peptide.)

Rabbit RGN/SMP30 Polyclonal Antibody | anti-RGN/SMP30 antibody

RGN/SMP30 Antibody

Gene Names
RGN; RC; GNL; SMP30; HEL-S-41
Reactivity
Human, Mouse, Rat
Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
RGN/SMP30; Polyclonal Antibody; RGN/SMP30 Antibody; Gluconolactonase; GNL; RC; Regucalcin; RGN; RGN_HUMAN; Senescence marker protein 30; SMP 30; SMP-30; anti-RGN/SMP30 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Clonality
Polyclonal
Isotype
IgG
Specificity
RGN/SMP30 Antibody detects endogenous levels of RGN/SMP30.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
299
Applicable Applications for anti-RGN/SMP30 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide derived from human RGN/SMP30.
Fragment
Fab Fragment
Conjugate
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Mouse liver tissue?, using RGN/SMP30 Antibody. The lane on the left was treated with blocking peptide.)

Western Blot (WB) (Western blot analysis of extracts from Mouse liver tissue?, using RGN/SMP30 Antibody. The lane on the left was treated with blocking peptide.)

Western Blot (WB)

(Western blot analysis of extracts from Mouse spleen, using RGN/SMP30 Antibody. The lane on the left was treated with blocking peptide.)

Western Blot (WB) (Western blot analysis of extracts from Mouse spleen, using RGN/SMP30 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-RGN/SMP30 antibody
Description: Gluconolactonase with low activity towards other sugar lactones, including gulonolactone and galactonolactone. Can also hydrolyze diisopropyl phosphorofluoridate and phenylacetate (in vitro). Calcium-binding protein. Modulates Ca2+ signaling, and Ca2+-dependent cellular processes and enzyme activities (By similarity).

Subunit Structure: Monomer.

Similarity: Belongs to the SMP-30/CGR1 family.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed: 33 kDa
Predicted: 34 kDa
NCBI Official Full Name
Regucalcin
NCBI Official Synonym Full Names
regucalcin
NCBI Official Symbol
RGN
NCBI Official Synonym Symbols
RC; GNL; SMP30; HEL-S-41
NCBI Protein Information
regucalcin
UniProt Protein Name
Regucalcin
UniProt Gene Name
RGN
UniProt Synonym Gene Names
SMP30; RC; GNL; SMP-30
UniProt Entry Name
RGN_HUMAN

NCBI Description

The protein encoded by this gene is a highly conserved, calcium-binding protein, that is preferentially expressed in the liver and kidney. It may have an important role in calcium homeostasis. Studies in rat indicate that this protein may also play a role in aging, as it shows age-associated down-regulation. This gene is part of a gene cluster on chromosome Xp11.3-Xp11.23. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

RGN: Gluconolactonase with low activity towards other sugar lactones, including gulonolactone and galactonolactone. Can also hydrolyze diisopropyl phosphorofluoridate and phenylacetate (in vitro). Calcium-binding protein. Modulates Ca(2+) signaling, and Ca(2+)-dependent cellular processes and enzyme activities. Belongs to the SMP-30/CGR1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.1.17

Chromosomal Location of Human Ortholog: Xp11.3

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: zinc ion binding; gluconolactonase activity; calcium ion binding; enzyme regulator activity

Biological Process: cellular calcium ion homeostasis; L-ascorbic acid biosynthetic process; positive regulation of ATPase activity; regulation of calcium-mediated signaling

Research Articles on RGN/SMP30

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Product Notes

The RGN/SMP30 rgn (Catalog #AAA9611471) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RGN/SMP30 Antibody reacts with Human, Mouse, Rat Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's RGN/SMP30 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the RGN/SMP30 rgn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RGN/SMP30, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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