GCAT recombinant protein

GCAT, 22-419aa, Human, His tag, E Coli (Denatured)

Cat. Num. AAA205301

• Gene Names: GCAT; KBL
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: GCAT; 22-419aa; Human; His tag; E Coli (Denatured); Glycine C-acetyltransferase; 2-amino-3-ketobutyrate coenzyme A ligase; mitochondrial; AKB ligase; Aminoacetone synthase; Glycine acetyltransferase; KBL; GCAT recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.4M urea, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MSALAQLRGI LEGELEGIRG AGTWKSERVI TSRQGPHIRV DGVSGGILNF CANNYLGLSS HPEVIQAGLQ ALEEFGAGLS SVRFICGTQS IHKNLEAKIA RFHQREDAIL YPSCYDANAG LFEALLTPED AVLSDELNHA SIIDGIRLCK AHKYRYRHLD MADLEAKLQE AQKHRLRLVA TDGAFSMDGD IAPLQEICCL ASRYGALVFM DECHATGFLG PTGRGTDELL GVMDQVTIIN STLGKALGGA SGGYTTGPGP LVSLLRQRAR PYLFSNSLPP AVVGCASKAL DLLMGSNTIV QSMAAKTQRF RSKMEAAGFT ISGASHPICP VMLGDARLAS RMADDMLKRG IFVIGFSYPV VPKGKARIRV QISAVHSEED IDRCVEAFVE VGRLHGALP
• Sequence Length: 419

• Applicable Applications for GCAT recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for GCAT recombinant protein

The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. GCAT is the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase. Recombinant human GCAT protein, fused to His-tag at N-terminus, was expressed in E Coli.

Product Categories/Family for GCAT recombinant protein

Metabolism

References

Edgar A.J., et al. (2000) Eur. J. Biochem. 267:1805-1812; Hoshino A., et al. (2007) Cell Stress Chaperones. 12:186-191

NCBI and Uniprot Product Information

• NCBI GI #: 7657118
• NCBI GeneID: 23464
• NCBI Accession #: NP_055106
• NCBI GenBank Nucleotide #: NM_014291.3
• UniProt Accession #: O75600; Q6ZWF1; Q96CA9; E2QC23
• Molecular Weight: 45.0kDa (419aa)
• NCBI Official Full Name: 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial isoform 2
• NCBI Official Synonym Full Names: glycine C-acetyltransferase
• NCBI Official Symbol: GCAT
• NCBI Official Synonym Symbols: KBL
• NCBI Protein Information: 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial
• UniProt Protein Name: 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial
• UniProt Gene Name: GCAT
• UniProt Synonym Gene Names: KBL; AKB ligase

NCBI Description

The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. This gene encodes the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The encoded enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 14. [provided by RefSeq, Jan 2010]