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Western Blot (WB) (Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Human RGS7 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Rabbit anti-Human Regulator Of G Protein Signaling 7 Polyclonal Antibody | anti-RGS7 antibody

Polyclonal Antibody to Regulator Of G Protein Signaling 7 (RGS7)

Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Regulator Of G Protein Signaling 7; Polyclonal Antibody; Polyclonal Antibody to Regulator Of G Protein Signaling 7 (RGS7); anti-RGS7 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against RGS7. It has been selected for its ability to recognize RGS7 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, Containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence Length
487
Applicable Applications for anti-RGS7 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Fragment
RGS7 (Met1~Glu144)
Conjugate
No Conjugate
Cross Reactivity
Human, Mouse, Rat
Immunoglobulin Type
IgG
Quality Control
Content: The quality control contains recombinant RGS7 (Met1~Glu144) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2072614
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Human RGS7 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB) (Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Human RGS7 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded kidney tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin- embedded kidney tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
56,741 Da
NCBI Official Full Name
regulator of G-protein signaling 7 isoform 2
NCBI Official Synonym Full Names
regulator of G protein signaling 7
NCBI Official Symbol
RGS7
NCBI Protein Information
regulator of G-protein signaling 7
UniProt Protein Name
Regulator of G-protein signaling 7
UniProt Gene Name
RGS7
UniProt Synonym Gene Names
RGS7

Uniprot Description

RGS7: inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Activity on G(o)-alpha is specifically enhanced by the RGS6/GNG5 dimer. May play a role in synaptic vesicle exocytosis. May play important role in the rapid regulation of neuronal excitability and the cellular responses to short-lived stimulations. Heterodimer with GNG5. Interacts with RGS7BP, leading to regulate the subcellular location of the heterodimer formed with Gbeta5 (By similarity). Interacts with 14-3-3 protein Tau and SNAP25BP. Four alternatively spliced isoforms have been described.

Protein type: GAP; GAP, RGS

Chromosomal Location of Human Ortholog: 1q43|1q23.1

Cellular Component: cytosol; heterotrimeric G-protein complex; plasma membrane

Molecular Function: G-protein beta-subunit binding; GTPase activator activity; signal transducer activity

Biological Process: G-protein coupled receptor signaling pathway; negative regulation of signal transduction; positive regulation of GTPase activity; protein folding

Research Articles on RGS7

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Product Notes

The RGS7 rgs7 (Catalog #AAA2033796) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Regulator Of G Protein Signaling 7 (RGS7) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Regulator Of G Protein Signaling 7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-1:5000. Researchers should empirically determine the suitability of the RGS7 rgs7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Regulator Of G Protein Signaling 7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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