Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using REG3G antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
Rabbit REG3G Polyclonal Antibody | anti-REG3G antibody
REG3G Polyclonal Antibody
Gene Names
Reg3g; AI449515
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Synonyms
REG3G; Polyclonal Antibody; REG3G Polyclonal Antibody; AI449515; anti-REG3G antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Sequence Length
174
Applicable Applications for anti-REG3G antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:10 - 1:100
IHC: 1:50 - 1:200
IF: 1:10 - 1:100
Species
Mouse
Immunogen
Recombinant Protein
Gene ID
130120
Immunogen
Recombinant protein of mouse REG3G
Calculated Molecular Weight
19kDa
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at -20 degree C (regular). Avoid freeze / thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using REG3G antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using REG3G antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
Immunofluorescence (IF)
(Immunofluorescence analysis of MCF-7 cells using REG3G antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of MCF-7 cells using REG3G antibody. Blue: DAPI for nuclear staining.)
Related Product Information for anti-REG3G antibody
The regeneration (REG) family consists of secretory proteins involved in liver, pancreatic, gastric and intestinal cell proliferation or differentiation. Members of the REG family are divided into four subclasses, designated types I, II, III and IV, all of which share a common gene structure containing five introns and six exons. Members of the Reg family have been implicated in the regulation of cell growt h, tumorigenesis and the progression of cancer. Reg lll© (regenerating islet - derived 3 gamma), also known as pancreatitis - associated protein 1B, PAP1B, or UNQ429, is a 175 amino acid secreted protein that is expressed almost exclusively in pancreas, with low levels of expression in testis. Reg lll© functions as an antimicrobial protein involved in controlling bacterial proliferation and may be induced during acute pancreatitis. The gene encoding Reg lll© maps to human chromosome 2, which houses over 1,400 g enes and comprises nearly 8% of the human genome
Product Categories/Family for anti-REG3G antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Regenerating islet-derived protein 3-gamma
NCBI Official Synonym Full Names
regenerating islet-derived 3 gamma
NCBI Official Symbol
Reg3g
NCBI Official Synonym Symbols
AI449515
NCBI Protein Information
regenerating islet-derived protein 3-gamma; REG-3-gamma; reg III-gamma; RegIII (gamma); pancreatitis-associated protein 3; regenerating islet-derived protein III-gamma; generating islet-derived, mouse homolog 3 gamma
UniProt Protein Name
Regenerating islet-derived protein 3-gamma
Protein Family
UniProt Gene Name
Reg3g
UniProt Synonym Gene Names
Pap3; REG-3-gamma; Reg III-gamma
UniProt Entry Name
REG3G_MOUSE
Uniprot Description
Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Restricts bacterial colonization of the intestinal epithelial surface and consequently limits activation of adaptive immune responses by the microbiota. The uncleaved form has bacteriostatic activity, whereas the cleaved form has bactericidal activity against L.monocytogenes and methicillin-resistant S.aureus. Regulates keratinocyte proliferation and differentiation after skin injury.
Research Articles on REG3G
Similar Products
Product Notes
The REG3G reg3g (Catalog #AAA126994) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The REG3G Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's REG3G can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:10 - 1:100. Researchers should empirically determine the suitability of the REG3G reg3g for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "REG3G, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
