REG3G recombinant protein

REG3G Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120895

• Gene Names: REG3G; PAP1B; PAPIB; PAP IB; PAP-1B; UNQ429; LPPM429; REG III; REG-III
• Purity: >97% as determined by SDS-PAGE
• Synonyms: REG3G; REG3G Protein; Human; Recombinant (His Tag); Human REG3G/PAP1B Protein (His Tag); LPPM429 Protein; PAP-1B Protein; PAP1B Protein; PAPIB Protein; REG-III Protein; REGIII Protein; UNQ429 Protein; regenerating islet-derived 3 gamma; REG3G recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asp175

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 27
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human REG3G (NP_001008388.1)(Met 1-Asp 175) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for REG3G recombinant protein

Background: Regenerating gene (Reg), first isolated from a regenerating islet cDNA library, encodes a secretory protein with a growth stimulating effect on pancreatic beta cells. Reg and Reg-related genes which were expressed in various organs have been revealed to constitute a multigene family, the Reg family, which consists of four subtypes (types I, II, III, IV) based on the primary structures of the encoded proteins of the genes, which are associated with tissue repair and have been directly implicated in pancreatic beta-cell regeneration. Reg proteins are expressed in various organs and are involved in cancers and neurodegenerative diseases. They display a typical C-type lectin-like domain but possess additional highly conserved amino acids. Regenerating islet-derived 3 gamma (REG3G), also known as pancreatitis-associated protein 1B (PAP1B), is a member of the secreted Reg superfamily and contains one typical C-type lectin domain. REG3G is expressed weakly in pancreas, strongly in intestinal tract, but not in hyperplastic islets REG3G might be a stress protein involved in the control of bacterial proliferation. It was indicated that REG3G specifically targets Gram-positive bacteria because it binds to their surface peptidoglycan layer, and serves as one of several antimicrobial peptides produced by paneth cells via stimulation of toll-like receptors (TLRs) by pathogen-associated molecular patterns (PAMPs).

References

Narushima Y, et al. (1997) Structure, chromosomal localization and expression of mouse genes encoding type III Reg, RegIII alpha, RegIII beta, RegIII gamma. Gene. 185(2): 159-68.Nata K, et al. (2004) Molecular cloning, expression and chromosomal localization of a novel human REG family gene, REG III. Gene. 340(1): 161-70.Laurine E, et al. (2005) PAP IB, a new member of the Reg gene family: cloning, expression, structural properties, and evolution by gene duplication. Biochim Biophys Acta. 1727(3): 177-87.Castellarin ML, et al. (2007) The identification and sequence analysis of a new Reg3gamma and Reg2 in the Syrian golden hamster. Biochim Biophys Acta. 1769(9-10): 579-85.

NCBI and Uniprot Product Information

• NCBI GI #: 56699413
• NCBI GeneID: 130120
• NCBI Accession #: NP_001008388.1
• NCBI GenBank Nucleotide #: NM_001008387.2
• UniProt Accession #: Q6UW15; Q3SYE4; Q3SYE6; Q6FH18; A8K980; D6W5J6
• Molecular Weight: 19,330 Da
• NCBI Official Full Name: regenerating islet-derived protein 3-gamma isoform 1
• NCBI Official Synonym Full Names: regenerating islet-derived 3 gamma
• NCBI Official Symbol: REG3G
• NCBI Official Synonym Symbols: PAP1B; PAPIB; PAP IB; PAP-1B; UNQ429; LPPM429; REG III; REG-III
• NCBI Protein Information: regenerating islet-derived protein 3-gamma; REG-3-gamma; reg III-gamma; regenerating gene III; pancreatitis-associated protein 1B; pancreatitis-associated protein IB; regenerating islet-derived protein III-gamma
• UniProt Protein Name: Regenerating islet-derived protein 3-gamma
• Protein Family: Regenerating islet-derived protein
• UniProt Gene Name: REG3G
• UniProt Synonym Gene Names: PAP1B; REG-3-gamma; PAP-1B; PAP IB; REG III
• UniProt Entry Name: REG3G_HUMAN

NCBI Description

This gene encodes a member of the regenerating islet-derived genes (REG)3 protein family. These proteins are secreted, C-type lectins with a carbohydrate recognition domain and N-terminal signal peptide. The protein encoded by this gene is an antibacterial lectin with activity against Gram-positive bacteria. Alternative splicing results in multiple transcript variants encoding multiple isoforms. [provided by RefSeq, Jun 2012]

Uniprot Description

REG3G: Might be a stress protein involved in the control of bacterial proliferation.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 2p12

Cellular Component: cytoplasm; extracellular region

Molecular Function: carbohydrate binding

Biological Process: MyD88-dependent toll-like receptor signaling pathway; defense response to Gram-positive bacterium; negative regulation of keratinocyte differentiation; acute-phase response