Mouse Regenerating Islet-Derived Protein 3-gamma (REG3G) ELISA Kit | REG3G elisa kit

Mouse Regenerating Islet-Derived Protein 3-gamma (REG3G) ELISA Kit

Cat. Num. AAA9717120

• Gene Names: Reg3g; AI449515; REG-3-gamma; reg III-gamma
• Reactivity: Mouse
• Synonyms: Regenerating Islet-Derived Protein 3-gamma (REG3G); Mouse Regenerating Islet-Derived Protein 3-gamma (REG3G) ELISA Kit; REG3G; MGC118998; MGC118999; MGC119001; PAP1B; PAPIB; REG-III; UNQ429; LPPM429; PAP IB protein; regenerating geneI; REG3G elisa kit

• Reactivity: Mouse
• Specificity: Mouse Regenerating islet-derived protein 3-gamma (REG3G) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse REG3G. No significant cross-reactivity or interference between Mouse REG3G and analogues was observed.
• Sequence Length: 174

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Mouse Regenerating islet-derived protein 3-gamma (REG3G) Standard Curve.)

Related Product Information for REG3G elisa kit

Description: This Mouse Regenerating islet-derived protein 3-gamma (REG3G) ELISA Kit employs a two-site sandwich ELISA to quantitate REG3G in samples. An antibody specific for REG3G has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any REG3G present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for REG3G is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of REG3G bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: REG3G encodes a deduced 175-amino acid protein, with a predicted molecular mass of 16.5 kD after cleavage of a putative 26-amino acid N-terminal signal peptide. Two REG3G splice forms, with identical coding sequences but different 5-prime UTRs, were identified. The REG3G and REG3A proteins share 85% sequence homology. Northern blot analysis and RT-PCR experiments showed that REG3G is strongly expressed in pancreas, moderately in testis, and weakly in heart, kidney, and placenta. Further RT-PCR analysis of pancreas-derived cell lines showed that REG3G expression is limited to exocrine pancreas. Using 3-dimensional protein structural modeling, they showed that although REG3G and REG1A share similar structural features, they display distinctive surface charge distributions.

NCBI and Uniprot Product Information

• NCBI GI #: 2811073
• NCBI GeneID: 19695
• UniProt Accession #: O09049
• NCBI Official Full Name: Regenerating islet-derived protein 3-gamma
• NCBI Official Synonym Full Names: regenerating islet-derived 3 gamma
• NCBI Official Symbol: Reg3g
• NCBI Official Synonym Symbols: AI449515; REG-3-gamma; reg III-gamma
• NCBI Protein Information: regenerating islet-derived protein 3-gamma
• UniProt Protein Name: Regenerating islet-derived protein 3-gamma
• Protein Family: Regenerating islet-derived protein
• UniProt Gene Name: Reg3g
• UniProt Synonym Gene Names: Pap3; REG-3-gamma; Reg III-gamma
• UniProt Entry Name: REG3G_MOUSE

NCBI Description

This gene encodes a C-type lectin that demonstrates bactericidal activity. This gene is predominantly expressed in the distal small intestine where the encoded protein undergoes proteolytic processing by trypsin. Mice lacking the encoded protein exhibit altered mucus distribution, increased bacterial contact with the epithelium, and elevated inflammatory markers in the ileum, and low-grade inflammation. [provided by RefSeq, Jun 2016]

Uniprot Description

Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Restricts bacterial colonization of the intestinal epithelial surface and consequently limits activation of adaptive immune responses by the microbiota. The uncleaved form has bacteriostatic activity, whereas the cleaved form has bactericidal activity against L.monocytogenes and methicillin-resistant S.aureus. Regulates keratinocyte proliferation and differentiation after skin injury.