Rabbit anti-Human CST3 Polyclonal Antibody | anti-CST3 antibody

CST3 (Cystatin C, ARMD11, MGC117328,CST3) (MaxLight 650)

Cat. Num. AAA6412743

• Gene Names: CST3; ARMD11
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Purified by affinity chromatography.
• Synonyms: CST3; Polyclonal Antibody; CST3 (Cystatin C; ARMD11; MGC117328; CST3) (MaxLight 650); anti-CST3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CST3.
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-CST3 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human CST3.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of extracts of various cells using 210291.)

Related Product Information for anti-CST3 antibody

Cystatin C is a 14kD member of the Cystatin superfamily of cysteine protease inhibitors (1). Most cell types secrete Cystatin C. Cystatin C inhibits cathepsins, and thereby may function as a tumor suppressor by inhibiting cathepsin mediated tumor cell invasion. In addition, this tumor suppressor function can also be attributed to Cystatin C's ability to antagonize TGF-beta1 signaling. Cystatin C may also modulate antigen presentation through its ability to inhibit cathepsins. Cystatin C is also a biomarker for kidney dysfunction.

Product Categories/Family for anti-CST3 antibody

Antibodies; Protease Inhibitors

NCBI and Uniprot Product Information

• NCBI GI #: 118183
• NCBI GeneID: 1471
• UniProt Accession #: P01034; Q6FGW9; B2R5J9; D3DW42
• Molecular Weight: 146
• NCBI Official Full Name: Cystatin-C
• NCBI Official Synonym Full Names: cystatin C
• NCBI Official Symbol: CST3
• NCBI Official Synonym Symbols: ARMD11
• NCBI Protein Information: cystatin-C; cystatin 3; cystatin-3; gamma-trace; post-gamma-globulin; bA218C14.4 (cystatin C); neuroendocrine basic polypeptide
• UniProt Protein Name: Cystatin-C
• Protein Family: Cystatin
• UniProt Gene Name: CST3
• UniProt Entry Name: CYTC_HUMAN

NCBI Description

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes the most abundant extracellular inhibitor of cysteine proteases, which is found in high concentrations in biological fluids and is expressed in virtually all organs of the body. A mutation in this gene has been associated with amyloid angiopathy. Expression of this protein in vascular wall smooth muscle cells is severely reduced in both atherosclerotic and aneurysmal aortic lesions, establishing its role in vascular disease. In addition, this protein has been shown to have an antimicrobial function, inhibiting the replication of herpes simplex virus. Alternative splicing results in multiple transcript variants encoding a single protein. [provided by RefSeq, Nov 2014]

Uniprot Description

CST3: As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. Defects in CST3 are the cause of amyloidosis type 6 (AMYL6); also known as hereditary cerebral hemorrhage with amyloidosis (HCHWA), cerebral amyloid angiopathy (CAA) or cerebroarterial amyloidosis Icelandic type. AMYL6 is a hereditary generalized amyloidosis due to cystatin C amyloid deposition. Cystatin C amyloid accumulates in the walls of arteries, arterioles, and sometimes capillaries and veins of the brain, and in various organs including lymphoid tissue, spleen, salivary glands, and seminal vesicles. Amyloid deposition in the cerebral vessels results in cerebral amyloid angiopathy, cerebral hemorrhage and premature stroke. Cystatin C levels in the cerebrospinal fluid are abnormally low. Genetic variations in CST3 are associated with age- related macular degeneration type 11 (ARMD11). ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. Belongs to the cystatin family.

Protein type: Secreted, signal peptide; Inhibitor; Secreted

Chromosomal Location of Human Ortholog: 20p11.21

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; protease binding; beta-amyloid binding; endopeptidase inhibitor activity; cysteine protease inhibitor activity

Biological Process: negative regulation of proteolysis; fibril organization and biogenesis; negative regulation of peptidase activity; defense response; regulation of tissue remodeling

Disease: Macular Degeneration, Age-related, 11; Cerebral Amyloid Angiopathy, Cst3-related