Rabbit anti-Human RCC2 Polyclonal Antibody | anti-RCC2 antibody

RCC2, ID (RCC2, KIAA1470, TD60, Protein RCC2, RCC1-like protein TD-60, Telophase disk protein of 60kD) (AP)

Cat. Num. AAA6341037

• Gene Names: RCC2; TD-60
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RCC2; Polyclonal Antibody; ID (RCC2; KIAA1470; TD60; Protein RCC2; RCC1-like protein TD-60; Telophase disk protein of 60kD) (AP); Telophase disk protein of 60kD; anti-RCC2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 522

• Applicable Applications for anti-RCC2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RCC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 169-198 amino acids from the Central region of human RCC2.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(RCC2 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the RCC2 antibody detected the RCC2 protein (arrow).)

Related Product Information for anti-RCC2 antibody

Required for completion of mitosis and cytokinesis. RCC2 may function as a guanine nucleotide exchange factor for the small GTPase RAC1.

Product Categories/Family for anti-RCC2 antibody

Antibodies; KIAA; orf

References

Humphries, J.D., et al. Sci Signal 2 (87), RA51 (2009): Stacey, S.N., et al. Nat. Genet. 40(11):1313-1318(2008). Matsuoka, S., et al. Science 316(5828):1160-1166(2007). Olsen, J.V., et al. Cell 127(3):635-648(2006). Olsen, J.V., et al. Cell 127(3):635-648(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 29789090
• NCBI GeneID: 55920
• NCBI Accession #: NP_061185.1
• NCBI GenBank Nucleotide #: NP_061185.1
• UniProt Accession #: Q9P258
• NCBI Official Full Name: protein RCC2
• NCBI Official Synonym Full Names: regulator of chromosome condensation 2
• NCBI Official Symbol: RCC2
• NCBI Official Synonym Symbols: TD-60
• NCBI Protein Information: protein RCC2
• UniProt Protein Name: Protein RCC2
• Protein Family: Protein
• UniProt Gene Name: RCC2
• UniProt Synonym Gene Names: KIAA1470; TD60
• UniProt Entry Name: RCC2_HUMAN

NCBI Description

The protein encoded by this gene is a guanine exchange factor that is active on RalA, a small GTPase. The encoded protein and RalA are both essential for proper kinetochore-microtubule function in early mitosis. This protein has been shown to be a biomarker for colorectal cancer. [provided by RefSeq, Oct 2016]

Uniprot Description

TD-60: Required for completion of mitosis and cytokinesis. May function as a guanine nucleotide exchange factor for the small GTPase RAC1. Binds preferentially to the nucleotide-free form of RAC1. Interacts with microtubules.

Protein type: GEFs, misc.; GEFs; Nucleolus

Chromosomal Location of Human Ortholog: 1p36.13

Cellular Component: microtubule; nucleolus; midbody; cytosol

Molecular Function: protein binding; microtubule binding; Rac GTPase binding

Biological Process: integrin-mediated signaling pathway; focal adhesion formation; mitosis; endosome organization and biogenesis; cell division; negative regulation of focal adhesion formation; positive regulation of attachment of spindle microtubules to kinetochore; mitotic cell cycle; regulation of cell migration