Rabbit RCC2 Polyclonal Antibody | anti-RCC2 antibody

RCC2 Polyclonal Antibody

Cat. Num. AAA3007613

• Gene Names: RCC2; TD-60
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: RCC2; Polyclonal Antibody; RCC2 Polyclonal Antibody; Protein RCC2; RCC1-like protein TD-60; Telophase disk protein of 60 kDa; KIAA1470; TD60; anti-RCC2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RCC2 polyclonal antibody detects endogenous levels of RCC2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 522

• Applicable Applications for anti-RCC2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 470-520 of Human RCC2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of RCC2 polyclonal antibody at 1:500 dilutionLane1:C6 whole cell lysate (40ug) Lane2:CT26 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:HCT116 whole cell lysate (40ug) Lane5:HepG2 whole cell lysate (40ug))

Related Product Information for anti-RCC2 antibody

RCC2/TD-60 is a member of the RCC1 (regulator of chromosome condensation 1) family of guanine nucleotide exchange factors. RCC2/TD-60 is associated with the chromosome passenger complex (CPC), which also consists of aurora B kinase, borealin, INCENP (inner centromere protein) and survivin. The CPC acts at various stages of mitosis, interacts with microtubules and is required for proper chromosome segregation and cytokinesis. Regulation of aurora B kinase is key in the regulation of the CPC. In late mitosis, RCC2/TD-60 is required for spindle assembly and recruitment of survivin and aurora B. RCC2/TD-60 is also required for aurora B activation in vitro and localization of the CPC to centromeres.

NCBI and Uniprot Product Information

• NCBI GI #: 209969703
• NCBI GeneID: 55920
• NCBI Accession #: NP_001129676.1
• NCBI GenBank Nucleotide #: NP_001129676.1
• UniProt Accession #: Q9P258
• Molecular Weight: ~ 60kDa
• NCBI Official Full Name: protein RCC2
• NCBI Official Synonym Full Names: regulator of chromosome condensation 2
• NCBI Official Symbol: RCC2
• NCBI Official Synonym Symbols: TD-60
• NCBI Protein Information: protein RCC2
• UniProt Protein Name: Protein RCC2
• Protein Family: Protein
• UniProt Gene Name: RCC2
• UniProt Synonym Gene Names: KIAA1470; TD60
• UniProt Entry Name: RCC2_HUMAN

NCBI Description

The protein encoded by this gene is a guanine exchange factor that is active on RalA, a small GTPase. The encoded protein and RalA are both essential for proper kinetochore-microtubule function in early mitosis. This protein has been shown to be a biomarker for colorectal cancer. [provided by RefSeq, Oct 2016]

Uniprot Description

TD-60: Required for completion of mitosis and cytokinesis. May function as a guanine nucleotide exchange factor for the small GTPase RAC1. Binds preferentially to the nucleotide-free form of RAC1. Interacts with microtubules.

Protein type: GEFs, misc.; GEFs; Nucleolus

Chromosomal Location of Human Ortholog: 1p36.13

Cellular Component: microtubule; nucleolus; midbody; cytosol

Molecular Function: protein binding; microtubule binding; Rac GTPase binding

Biological Process: integrin-mediated signaling pathway; focal adhesion formation; mitosis; endosome organization and biogenesis; cell division; negative regulation of focal adhesion formation; positive regulation of attachment of spindle microtubules to kinetochore; mitotic cell cycle; regulation of cell migration