Rabbit RBPMS Polyclonal Antibody | anti-RBPMS antibody

Anti-RBPMS

Cat. Num. AAA502220

• Gene Names: Rbpms; RBP-MS; hermes; AU017537; 2010300K22Rik; 2700019M19Rik
• Reactivity: Canine, Hamster
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity purification via chromatography on an affinity column prepared with the peptide used as antigen.
• Synonyms: RBPMS; Polyclonal Antibody; Anti-RBPMS; anti-RBPMS antibody

• Host: Rabbit
• Reactivity: Canine, Hamster
• Clonality: Polyclonal
• Specificity: Specific for endogenous levels of the ~24kDa RBPMS protein
• Purity/Purification: Affinity purification via chromatography on an affinity column prepared with the peptide used as antigen.
• Form/Format: 100 ul antibody per vial in PBS + 0.03% NaN₃. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 197

• Applicable Applications for anti-RBPMS antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocystochemistry (ICC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1,000; IHC: 1:100-1:2,000; ICC: 1:200-1:300; FC/FACS: 1:400
• Antigen: Synthetic peptide corresponding to amino acid residues from the N-terminal region of the rat RBPMS sequence conjugated to KLH.
• Preparation and Storage: Store at -20 degree C.

Immunohistochemistry (IHC)

(IHC of mouse retinal ganglion cells showing specific immunolabeling of RBPMS in red.)

Western Blot (WB)

(Western blot of rat heart lysate showing specific labeling of the ~24 kDaRBPMS protein.)

Related Product Information for anti-RBPMS antibody

RBPMS (RNA binding protein with multiple splicing), also known as HERMES, contains one RRM (RNA recognition motif) domain and belongs to the RRM family of RNA-binding proteins. RBPMS exists as multiple alternatively spliced isoforms and is thought to bind RNA, possibly playing a role in RNA-related events, such as transcription and translation. RNA-binding proteins that are specific to retinal ganglion cells (RGCs) have been previously identified as excellent markers for RGCs (Kwong et al., 2010). Recent findings show that antibodies against RBPMS are robust reagents that exclusively identify RGCs in multiple mammalian species (Rodriguez et al. 2014).

NCBI and Uniprot Product Information

• NCBI GI #: 111185961
• NCBI GeneID: 19663
• NCBI Accession #: NP_001036139.1
• NCBI GenBank Nucleotide #: NM_001042674.2
• UniProt Accession #: Q9WVB0; Q78HG1; Q9CPU5
• Molecular Weight: ~24kDa
• NCBI Official Full Name: RNA-binding protein with multiple splicing isoform 1
• NCBI Official Synonym Full Names: RNA binding protein gene with multiple splicing
• NCBI Official Symbol: Rbpms
• NCBI Official Synonym Symbols: RBP-MS; hermes; AU017537; 2010300K22Rik; 2700019M19Rik
• NCBI Protein Information: RNA-binding protein with multiple splicing
• UniProt Protein Name: RNA-binding protein with multiple splicing
• Protein Family: RNA-binding protein with multiple splicing
• UniProt Gene Name: Rbpms
• UniProt Synonym Gene Names: Hermes; RBP-MS; Hermes
• UniProt Entry Name: RBPMS_MOUSE

Uniprot Description

Acts as a coactivator of transcriptional activity. Required to increase TGFB1/Smad-mediated transactivation. Acts through SMAD2, SMAD3 and SMAD4 to increase transcriptional activity. Increases phosphorylation of SMAD2 and SMAD3 on their C-terminal SSXS motif, possibly through recruitment of TGFBR1. Promotes the nuclear accumulation of SMAD2, SMAD3 and SMAD4 proteins. Binds to poly(A) RNA.