Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cell using PHC3 antibody. Blue: DAPI for nuclear staining.)
Rabbit anti-Human, Mouse PHC3 Polyclonal Antibody | anti-PHC3 antibody
PHC3 antibody
Gene Names
PHC3; EDR3; HPH3
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence
Purity
Antibodies were purified by affinity purification using immunogen.
Synonyms
PHC3; Polyclonal Antibody; PHC3 antibody; EDR3; HPH3; anti-PHC3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
The antibody detects endogenous level of total PHC3 antibody.
Purity/Purification
Antibodies were purified by affinity purification using immunogen.
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
983
Applicable Applications for anti-PHC3 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
Western blotting: 1:500 - 1:2000
Immunofluorescence: 1:50 - 1:100
Immunofluorescence: 1:50 - 1:100
Immunogen Type
Recombinant Protein
Immunogen Description
Recombinant protein of human PHC3.
Target Name
PHC3
Preparation and Storage
Store at -20 degree C
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cell using PHC3 antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cell using PHC3 antibody. Blue: DAPI for nuclear staining.)
Related Product Information for anti-PHC3 antibody
Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
Product Categories/Family for anti-PHC3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
107,480 Da
NCBI Official Full Name
polyhomeotic-like protein 3 isoform 2
NCBI Official Synonym Full Names
polyhomeotic homolog 3 (Drosophila)
NCBI Official Symbol
PHC3
NCBI Official Synonym Symbols
EDR3; HPH3
NCBI Protein Information
polyhomeotic-like protein 3
UniProt Protein Name
Polyhomeotic-like protein 3
Protein Family
UniProt Gene Name
PHC3
UniProt Synonym Gene Names
EDR3; PH3; hPH3
UniProt Entry Name
PHC3_HUMAN
Uniprot Description
PHC3: Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. 7 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription regulation
Chromosomal Location of Human Ortholog: 3q26.2
Cellular Component: nucleoplasm; intracellular membrane-bound organelle; PcG protein complex; nucleus; actin cytoskeleton
Molecular Function: DNA binding; zinc ion binding
Biological Process: multicellular organismal development
Research Articles on PHC3
Similar Products
Product Notes
The PHC3 phc3 (Catalog #AAA9407715) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PHC3 antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PHC3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). Western blotting: 1:500 - 1:2000 Immunofluorescence: 1:50 - 1:100. Researchers should empirically determine the suitability of the PHC3 phc3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PHC3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
