Rabbit anti-Human B4GALNT1 Polyclonal Antibody | anti-B4GALNT1 antibody

Anti-B4GALNT1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8108477

• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: B4GALNT1; Polyclonal Antibody; Anti-B4GALNT1 Antibody; Rabbit Polyclonal; B4GALNT1 Antibody; Rabbit PAb; Antigen Affinity Purified; beta-1; 4-N-acetyl-galactosaminyl transferase 1; Anti-GALGT Antibody; Anti-GalNAc-T Antibody; Anti-GALNACT Antibody; Anti-SPG26 Antibody; anti-B4GALNT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human B4GALNT1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-B4GALNT1 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:50-1:200
• Immunogen: E Coli-derived Human B4GALNT1 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human B4GALNT1 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human B4GALNT1 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-B4GALNT1 antibody

BGNT-1.1 in ciliated sensory neuron function and morphogenesis. BGNT-1.1 functions at the trans-Golgi network of sheath cells (glia) to influence dye-filling and cilium length, in a cell non-autonomous manner. Notably, BGNT-1.1 is the orthologue of human B3GNT1/B4GAT1, a glycosyltransferase associated with Walker-Warburg syndrome (WWS). WWS is a multigenic disorder characterised by muscular dystrophy as well as brain and eye anomalies. Dystroglycan is a cell membrane receptor that organizes the basement membrane by binding ligands in the extracellular matrix. Proper glycosylation of the alpha-dystroglycan (alpha-DG) subunit is essential for these activities, and lack thereof results in neuromuscular disease. we had known that FKRP, FKTN, TMEM5 and B4GAT1 (formerly known as B3GNT1) localize to the Golgi and contribute to the O-mannosyl post-phosphorylation modification of alpha-DG. Moreover, we assigned B4GAT1 a function as a xylose beta1, 4-glucuronyltransferase. Nuclear magnetic resonance studies confirmed that a glucuronic acid beta1, 4-xylose disaccharide synthesized by B4GAT1 acts as an acceptor primer that can be elongated by LARGE with the ligand-binding heteropolysaccharide. Recent studies demonstrated that mutations in B3GNT1, an enzyme proposed to be involved in poly-N-acetyllactosamine synthesis, were causal for congenital muscular dystrophy with hypoglycosylation of alpha-dystroglycan (secondary dystroglycanopathies). Since defects in the O-mannosylation protein glycosylation pathway are primarily responsible for dystroglycanopathies and with no established O-mannose initiated structures containing a beta3 linked GlcNAc known, we biochemically interrogated this human enzyme.