Mouse anti-Human RBM4 Monoclonal Antibody | anti-RBM4 antibody

RBM4 Monoclonal Antibody

Cat. Num. AAA2542047

• Gene Names: RBM4; LARK; RBM4A; ZCRB3A; ZCCHC21
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
• Purity: Protein A purification
• Synonyms: RBM4; Monoclonal Antibody; RBM4 Monoclonal Antibody; dkfzp547k0918; hLark; lark; Lark homolog; lark homologue; mgc75138; rbm4 lark; RNA binding motif 4; RNA binding motif 4a; RNA-binding motif protein 4; RNA-binding motif protein 4a; RNA-binding protein 4; anti-RBM4 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 634
• Purity/Purification: Protein A purification
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 143

• Applicable Applications for anti-RBM4 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: WB: 1:500-1:5000; IHC: 1:20-1:200; IF: 1:50-1:500
• Immunogen: Fusion protein of RBM4
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, PH7.3
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western Blot analysis of HeLa cells using RBM4 Monoclonal Antibody at dilution of 1:1000)

Related Product Information for anti-RBM4 antibody

RBM4, RNA-binding motif protein4, contains 2 RRM-type RNA-binding motif and a retroviral-type(RT) zinc finger. RNA-binding factor participates in number of aspects of cellular processes such as alternative splicing of pre-mRNA and translation regulation. RBM4 recruits eIF4A1 to stimulate IRES-dependent translation in responds to cellular stress. Once assembled at the rHRE, the HIF2A-RBM4-eIF4E2 complex captures the 5-prime cap and targets mRNAs to polysomes for active translation, thereby evading hypoxia-induced repression of protein synthesis.

Product Categories/Family for anti-RBM4 antibody

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 311771540
• NCBI GeneID: 5936
• NCBI Accession #: NP_001185772.1
• NCBI GenBank Nucleotide #: NM_001198843.1
• UniProt Accession #: Q9BWF3; O02916; Q4VC48; Q6P1P2; Q8WU85; B3KUN0; B4E1U0; E7EQS3
• Molecular Weight: Calculated: 20kDa; 40kDa; Observed: 40kDa
• NCBI Official Full Name: RNA-binding protein 4 isoform 2
• NCBI Official Synonym Full Names: RNA binding motif protein 4
• NCBI Official Symbol: RBM4
• NCBI Official Synonym Symbols: LARK; RBM4A; ZCRB3A; ZCCHC21
• NCBI Protein Information: RNA-binding protein 4
• UniProt Protein Name: RNA-binding protein 4
• Protein Family: RNA-binding protein
• UniProt Gene Name: RBM4
• UniProt Synonym Gene Names: RBM4A; hLark

Uniprot Description

RNA-binding factor involved in multiple aspects of cellular processes like alternative splicing of pre-mRNA and translation regulation. Modulates alternative 5'-splice site and exon selection. Acts as a muscle cell differentiation-promoting factor. Activates exon skipping of the PTB pre-mRNA during muscle cell differentiation. Antagonizes the activity of the splicing factor PTBP1 to modulate muscle cell-specific exon selection of alpha tropomyosin. Binds to intronic pyrimidine-rich sequence of the TPM1 and MAPT pre-mRNAs. Required for the translational activation of PER1 mRNA in response to circadian clock. Binds directly to the 3'-UTR of the PER1 mRNA. Exerts a suppressive activity on Cap-dependent translation via binding to CU-rich responsive elements within the 3'UTR of mRNAs, a process increased under stress conditions or during myocytes differentiation. Recruits EIF4A1 to stimulate IRES-dependent translation initiation in respons to cellular stress. Associates to internal ribosome entry segment (IRES) in target mRNA species under stress conditions. Plays a role for miRNA-guided RNA cleavage and translation suppression by promoting association of AGO2-containing miRNPs with their cognate target mRNAs. Associates with miRNAs during muscle cell differentiation. Binds preferentially to 5'-CGCGCG[GCA]-3' motif in vitro.