Immunofluorescence (IF)
(MBS9605844 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Rabbit anti-Human, Mouse RASL10A Polyclonal Antibody | anti-RASL10A antibody
RASL10A Antibody
Gene Names
RASL10A; RRP22
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
RASL10A; Polyclonal Antibody; RASL10A Antibody; Ras like protein family member 10A; Ras like protein RRP22; RAS like; family 10; member A; RAS related on chromosome 22; Ras related protein on chromosome 22; Ras related protein on chromosome 22 homolog; Ras-like protein family member 10A; Ras-like protein RRP22; Ras-related protein on chromosome 22; RASL 10A; Rasl10a; RASL10A protein; Rasl10a RAS like; RRP 22; RRP22; RSLAA_HUMAN; anti-RASL10A antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
RASL10A antibody detects endogenous levels of total RASL10A
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
203
Applicable Applications for anti-RASL10A antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Plasma Membrane; Nucleus
Tissue Specificity
Expression appears to be strictly limited to the central nervous system.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Dog
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9605844 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9605844 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using MRPS7 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using MRPS7 antibody.)
Related Product Information for anti-RASL10A antibody
Function: Potent inhibitor of cellular proliferation.
Post-translational Modifications: Isoprenylation is essential for nucleolar localization, and the proliferation-inhibiting activity of RASL10A.
Similarity: Belongs to the small GTPase superfamily. Ras family.
Post-translational Modifications: Isoprenylation is essential for nucleolar localization, and the proliferation-inhibiting activity of RASL10A.
Similarity: Belongs to the small GTPase superfamily. Ras family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 30 kD
Predicted: 23 kDa
Predicted: 23 kDa
NCBI Official Full Name
ras-like protein family member 10A
NCBI Official Synonym Full Names
RAS like family 10 member A
NCBI Official Symbol
RASL10A
NCBI Official Synonym Symbols
RRP22
NCBI Protein Information
ras-like protein family member 10A
UniProt Protein Name
Ras-like protein family member 10A
Protein Family
UniProt Gene Name
RASL10A
UniProt Synonym Gene Names
RRP22
Uniprot Description
Potent inhibitor of cellular proliferation.
Research Articles on RASL10A
Similar Products
Product Notes
The RASL10A rasl10a (Catalog #AAA9605844) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RASL10A Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RASL10A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the RASL10A rasl10a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RASL10A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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