Hypoxia Inducible Factor 1 Alpha (HIF1a) Recombinant Protein | HIF1a recombinant protein

Recombinant Hypoxia Inducible Factor 1 Alpha (HIF1a)

Cat. Num. AAA2010345

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Hypoxia Inducible Factor 1 Alpha (HIF1a); Recombinant Hypoxia Inducible Factor 1 Alpha (HIF1a); HIF1a recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-ASPYSGNRSR TASPIRAGKG TLEQTEKSCP GAPSLITVTL NKRSTAMDEE LNPKMLALHN AQRKRKMEHD GSLFQAVGIG SLFQQTGDRG GNASLAWKRV KACKTNGHNG VEQKTIILLS TDIASKLLGQ SMDESGLPQL TSYDC
• Sequence Length: 811

• Applicable Applications for HIF1a recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Chicken (Gallus)
• Expression System: Prokaryotic expression
• Residues: Ala641~Cys785 (Accession # Q9YIB9) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 45383550
• NCBI GeneID: 374177
• NCBI Accession #: NP_989628.1
• NCBI GenBank Nucleotide #: NM_204297.1
• UniProt Accession #: Q9YIB9
• Molecular Weight: 17.1kDa
• NCBI Official Full Name: hypoxia-inducible factor 1-alpha
• NCBI Official Synonym Full Names: hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
• NCBI Official Symbol: HIF1A
• NCBI Protein Information: hypoxia-inducible factor 1-alpha; HIF1-alpha; HIF-1-alpha; hypoxia-inducible factor 1 alpha; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
• UniProt Protein Name: Hypoxia-inducible factor 1-alpha
• Protein Family: Hypoxia-inducible factor
• UniProt Gene Name: HIF1A
• UniProt Synonym Gene Names: HIF-1-alpha; HIF1-alpha
• UniProt Entry Name: HIF1A_CHICK

Uniprot Description

Function: Functions as a master transcriptional regulator of the adaptive response to hypoxia. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators

By similarity.

Subunit structure: Efficient DNA binding requires heterodimerization of an alpha and a beta/ARNT subunit

By similarity.

Subcellular location: Cytoplasm

By similarity. Nucleus

By similarity. Note: Cytoplasmic in normoxia, nuclear translocation in response to hypoxia

By similarity.

Domain: Contains two independent C-terminal transactivation domains, NTAD and CTAD, which function synergistically. Their transcriptional activity is repressed by an intervening inhibitory domain (ID)

By similarity.

Post-translational modification: In normoxia, is hydroxylated on Pro-402 and Pro-562. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization

By similarity.In normoxia, is hydroxylated on Asn-788, thus abrogating interaction with CREBBP and EP300 and preventing transcriptional activation

By similarity.The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains

By similarity.

Sequence similarities: Contains 1 bHLH (basic helix-loop-helix) domain.Contains 1 PAC (PAS-associated C-terminal) domain.Contains 2 PAS (PER-ARNT-SIM) domains.