Western Blot (WB)
(Western Blot detection of SWAP70 in Hela, 3T3, CHO-K1 and C6 cell lysates and using SWAP70 antibody (1:500 diluted).)
Mouse SWAP70 Monoclonal Antibody | anti-SWAP70 antibody
SWAP70 Monoclonal Antibody
Reactivity
Human, Mouse, Rat, Hamster
Applications
Western Blot, Immunoprecipitation
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
SWAP70; Monoclonal Antibody; SWAP70 Monoclonal Antibody; Switch-associated protein 70; HSPC321; SWAP-70; FLJ39540; KIAA0640; anti-SWAP70 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat, Hamster
Clonality
Monoclonal
Specificity
This antibody detects endogenous levels of SWAP70 and does not cross-react with related proteins
Purity/Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
585
Applicable Applications for anti-SWAP70 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1000
IP: 1:50-100
IP: 1:50-100
Immunogen
Recombinant full length Human SWAP70.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot detection of SWAP70 in Hela, 3T3, CHO-K1 and C6 cell lysates and using SWAP70 antibody (1:500 diluted).)
Western Blot (WB)
(Western Blot detection of SWAP70 in Hela, 3T3, CHO-K1 and C6 cell lysates and using SWAP70 antibody (1:500 diluted).)
Immunoprecipitation (IP)
(Immunoprecipitation analysis of Hela cell lysate using SWAP70 antibody.)
Immunoprecipitation (IP)
(Immunoprecipitation analysis of Hela cell lysate using SWAP70 antibody.)
Related Product Information for anti-SWAP70 antibody
Phosphatidylinositol 3, 4, 5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3, 4)-bisphosphate production and cell protrusion
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted Band Size: 70KDa
Observed Band Size: 70KDa
Observed Band Size: 70KDa
NCBI Official Full Name
switch-associated protein 70 isoform 1
UniProt Protein Name
Switch-associated protein 70
Protein Family
UniProt Gene Name
SWAP70
UniProt Synonym Gene Names
KIAA0640; SWAP-70
UniProt Entry Name
SWP70_HUMAN
Uniprot Description
SWAP-70: Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion.
Protein type: GEFs; GEFs, Rac/Rho
Chromosomal Location of Human Ortholog: 11p15
Cellular Component: lamellipodium; cytoplasm; plasma membrane; nucleus
Molecular Function: DNA binding; calcium ion binding; ATP binding
Biological Process: isotype switching
Similar Products
Product Notes
The SWAP70 swap70 (Catalog #AAA3014341) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SWAP70 Monoclonal Antibody reacts with Human, Mouse, Rat, Hamster and may cross-react with other species as described in the data sheet. AAA Biotech's SWAP70 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1000 IP: 1:50-100. Researchers should empirically determine the suitability of the SWAP70 swap70 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SWAP70, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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