Western Blot (WB)
(Western Blot analysis of RanGAP1 on different cell lysates using anti-RanGAP1 antibody at 1/1,000 dilution. Positive control:Lane 1: MCF-7 Lane 2: SiHa Lane 3: 293)
Rabbit RanGAP1 Polyclonal Antibody | anti-RanGAP1 antibody
RanGAP1 Polyclonal Antibody
Gene Names
RANGAP1; SD; Fug1; RANGAP
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation
Purity
ProA affinity purified
Synonyms
RanGAP1; Polyclonal Antibody; RanGAP1 Polyclonal Antibody; RANGAP1; KIAA1835; SD; Fug 1; Fug1; GTPase-activating protein; RAN; 1; MGC20266; OTTHUMP00000028918; OTTHUMP00000198755; OTTHUMP00000198756; OTTHUMP00000198757; OTTHUMP00000198758; RAGP1; Ran 1; RAN GTPase activating protein 1; Ran GTPase-activating protein 1; Ran1; RANGAP 1; RANGAP; Segregation distorter homolog; Segregation distortion; anti-RanGAP1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
RanGAP1 polyclonal antibody detects endogenous levels of RanGAP1 protein.
Purity/Purification
ProA affinity purified
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
587
Applicable Applications for anti-RanGAP1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2,000
ICC: 1:100
IF: 1:100
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
IP: 1:10-1:50
ICC: 1:100
IF: 1:100
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
IP: 1:10-1:50
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C.
Avoid repeated freeze/thaw cycles.
Aliquot store at -20 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of RanGAP1 on different cell lysates using anti-RanGAP1 antibody at 1/1,000 dilution. Positive control:Lane 1: MCF-7 Lane 2: SiHa Lane 3: 293)
Western Blot (WB)
(Western Blot analysis of RanGAP1 on different cell lysates using anti-RanGAP1 antibody at 1/1,000 dilution. Positive control:Lane 1: MCF-7 Lane 2: SiHa Lane 3: 293)
Immunocytochemistry (ICC)
(ICC staining RanGAP1 (green) in 293T cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining RanGAP1 (green) in 293T cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Related Product Information for anti-RanGAP1 antibody
The small Ras related protein Ran, also called TC4, is a nuclear localized GTPase implicated in a diverse array of cellular processes including DNA replication, entry into and exit from mitosis and the transport of RNA and proteins through the nuclear pore complex. Like Ras, active Ran GTP and inactive Ran GDP levels are tightly regulated by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). The abundant GEF RCC1 (regulator of chromosome condensation 1) increases the rate at which Ran exchanges GDP for GTP. Ran GAP1 opposes the effects of RCC1 by increasing the rate at which Ran hydrolyzes GTP to GDP. A protein designated Ran BP1 has no intrinsic GAP activity and functions as a GEF inhibitor deactivating RCC1 and thereby indirectly increasing the ratio of Ran GDP to Ran GTP. Ran BP2 has been proposed as the Ran GTP docking site at the periphery of the nuclear pore complex.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
64kDa
NCBI Official Full Name
ran GTPase-activating protein 1
NCBI Official Synonym Full Names
Ran GTPase activating protein 1
NCBI Official Symbol
RANGAP1
NCBI Official Synonym Symbols
SD; Fug1; RANGAP
NCBI Protein Information
ran GTPase-activating protein 1
UniProt Protein Name
Ran GTPase-activating protein 1
Protein Family
UniProt Gene Name
RANGAP1
UniProt Synonym Gene Names
KIAA1835; SD
UniProt Entry Name
RAGP1_HUMAN
NCBI Description
This gene encodes a protein that associates with the nuclear pore complex and participates in the regulation of nuclear transport. The encoded protein interacts with Ras-related nuclear protein 1 (RAN) and regulates guanosine triphosphate (GTP)-binding and exchange. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Research Articles on RanGAP1
Similar Products
Product Notes
The RanGAP1 rangap1 (Catalog #AAA3013342) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RanGAP1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RanGAP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunoprecipitation (IP). WB: 1:500-1:2,000 ICC: 1:100 IF: 1:100 IHC: 1:50-1:200 FC/FACS: 1:50-1:100 IP: 1:10-1:50. Researchers should empirically determine the suitability of the RanGAP1 rangap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RanGAP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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