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Western Blot (WB)
(Western blot analysis of RanGAP1 on different cell lysates using anti-RanGAP1 antibody at 1/1, 000 dilution. Positive control: Lane 1: MCF-7 Lane 2: SiHa Lane 3: 293)
Rabbit RanGAP1 Monoclonal Antibody | anti-RanGAP1 antibody
RanGAP1 Antibody
Gene Names
RANGAP1; SD; Fug1; KIAA1835; MGC20266; RANGAP1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation
Purity
ProA affinity purified
Synonyms
RanGAP1; Monoclonal Antibody; RanGAP1 Antibody; Fug 1 antibody; Fug1 antibody; GTPase-activating protein; RAN; 1 antibody; KIAA1835 antibody; MGC20266 antibody; OTTHUMP00000028918 antibody; OTTHUMP00000198755 antibody; OTTHUMP00000198756 antibody; OTTHUMP00000198757 antibody; OTTHUMP00000198758 antibody; RAGP1_HUMAN antibody; Ran 1 antibody; RAN GTPase activating protein 1 antibody; Ran GTPase-activating protein 1 antibody; Ran1 antibody; RANGAP 1 antibody; RANGAP antibody; RanGAP1 antibody; SD antibody; Segregation distorter homolog antibody; Segregation distortion antibody; anti-RanGAP1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JG37-41
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-RanGAP1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF),Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:100
IP: 1:10-1:50
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
ICC/IF: 1:100
IP: 1:10-1:50
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western blot analysis of RanGAP1 on different cell lysates using anti-RanGAP1 antibody at 1/1, 000 dilution. Positive control: Lane 1: MCF-7 Lane 2: SiHa Lane 3: 293)
Western Blot (WB)
(Western blot analysis of RanGAP1 on different cell lysates using anti-RanGAP1 antibody at 1/1, 000 dilution. Positive control: Lane 1: MCF-7 Lane 2: SiHa Lane 3: 293)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-RanGAP1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-RanGAP1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-RanGAP1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-RanGAP1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-RanGAP1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-RanGAP1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-RanGAP1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-RanGAP1 antibody. Counter stained with hematoxylin.)
Immunocytochemistry (ICC)
(ICC staining RanGAP1 (green) in 293T cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining RanGAP1 (green) in 293T cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of A431 cells with RanGAP1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of A431 cells with RanGAP1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Related Product Information for anti-RanGAP1 antibody
The small Ras related protein Ran, also called TC4, is a nuclear localized GTPase implicated in a diverse array of cellular processes including DNA replication, entry into and exit from mitosis and the transport of RNA and proteins through the nuclear pore complex. Like Ras, active Ran GTP and inactive Ran GDP levels are tightly regulated by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). The abundant GEF RCC1 (regulator of chromosome condensation 1) increases the rate at which Ran exchanges GDP for GTP. Ran GAP1 opposes the effects of RCC1 by increasing the rate at which Ran hydrolyzes GTP to GDP. A protein designated Ran BP1 has no intrinsic GAP activity and functions as a GEF inhibitor deactivating RCC1 and thereby indirectly increasing the ratio of Ran GDP to Ran GTP. Ran BP2 has been proposed as the Ran GTP docking site at the periphery of the nuclear pore complex.
Product Categories/Family for anti-RanGAP1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
ran GTPase-activating protein 1 [Homo sapiens]
NCBI Official Synonym Full Names
Ran GTPase activating protein 1
NCBI Official Symbol
RANGAP1
NCBI Official Synonym Symbols
SD; Fug1; KIAA1835; MGC20266; RANGAP1
UniProt Protein Name
Ran GTPase-activating protein 1
Protein Family
UniProt Gene Name
RANGAP1
UniProt Synonym Gene Names
KIAA1835; SD
UniProt Entry Name
RAGP1_HUMAN
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Product Notes
The RanGAP1 rangap1 (Catalog #AAA9436655) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RanGAP1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RanGAP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF),Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC: 1:50-1:200 ICC/IF: 1:100 IP: 1:10-1:50 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the RanGAP1 rangap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RanGAP1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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