Endoglin/CD105 Active Protein | ENG active protein

Endoglin/CD105 Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8120027

• Gene Names: ENG; END; HHT1; ORW1
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Endoglin/CD105; Endoglin/CD105 Protein; Human; Recombinant (hFc Tag); Human Endoglin/CD105/ENG Protein (Fc Tag); END Protein; HHT1 Protein; ORW1 Protein; endoglin; ENG active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gly586

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 26
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the extracellular domain (Met 1-Gly 586) of human CD105 (NP_001108225.1) precursor was expressed with C-terminal fused human IgG1 Fc region.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: 1. Measured by its ability to bind biotinylated Human TGFRB1 in a functional ELISA.; 2. Measured by its ability to bind biotinylated Human ALK1-Fc in functional Elisa.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for ENG active protein

Background: Endoglin, also known as CD15, is a type I homodimeric transmembrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated cytoplasmic tail. Endoglin contains an RGD tripeptide which is a key recognition structure in cellular adhesion,,suggesting a critical role for endoglin in the binding of endothelial cells to integrins and/or other RGD receptors. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, mesenchymal stem cells and leukemic cells of lymphoid and myeloid lineages. As an accessory receptor for the TGF-beta superfamily ligands, endoglin binds TGF-beta1 and TGF-beta3 with high affinity not by itself but by associating with TGF-beta type II receptor (TbetaRII) and activates the downstream signal pathways. In addition, in human umbilical vein endothelial cells, ALK-1 is also a receptor kinase for endoglin threonine phosphorylation, and mutations in either of the two genes result in the autosomal-dominant vascular dysplasia, hereditary hemorrhagic telangiectasia (HHT). Endoglin has been regarded as a powerful biomarker of neovascularization, and is associated with several solid tumor types.

Product Categories/Family for ENG active protein

Hemangioblast Markers Cancer Drug Targets

References

Bellon T., et al.,(1993), Identification and expression of two forms of the human transforming growth factor-beta-binding protein endoglin with distinct cytoplasmic regions. Eur. J. Immunol. 23:2340-2345.Humphray S.J., et al., (2004), DNA sequence and analysis of human chromosome 9.Nature 429:369-374.Gougos A., et al.,(1990), Primary structure of endoglin, an RGD-containing glycoprotein of human endothelial cells.J. Biol. Chem. 265:8361-8364.

NCBI and Uniprot Product Information

• NCBI GI #: 168693647
• NCBI GeneID: 2022
• NCBI Accession #: NP_001108225.1
• NCBI GenBank Nucleotide #: NM_001114753.2
• Molecular Weight: 67,542 Da
• NCBI Official Full Name: endoglin isoform 1
• NCBI Official Synonym Full Names: endoglin
• NCBI Official Symbol: ENG
• NCBI Official Synonym Symbols: END; HHT1; ORW1
• NCBI Protein Information: endoglin; CD105 antigen
• Protein Family: Endoglin

NCBI Description

This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds to the beta1 and beta3 peptides with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia. This gene may also be involved in preeclampsia and several types of cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013]