Western Blot (WB)
(RANGAP1 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the RANGAP1 antibody detected the RANGAP1 protein (arrow).)
Rabbit anti-Human RANGAP1 Polyclonal Antibody | anti-RANGAP1 antibody
RANGAP1, NT (RANGAP1, KIAA1835, SD, Ran GTPase-activating protein 1)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Western Blot (WB)
(RANGAP1 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the RANGAP1 antibody detected the RANGAP1 protein (arrow).)
Western Blot (WB)
(RANGAP1 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the RANGAP1 antibody detected the RANGAP1 protein (arrow).)
Western Blot (WB)
(Western blot analysis of RANGAP1 (arrow) using rabbit polyclonal RANGAP1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RANGAP1 gene.)
Western Blot (WB)
(Western blot analysis of RANGAP1 (arrow) using rabbit polyclonal RANGAP1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RANGAP1 gene.)
Immunohistochemistry (IHC)
(RANGAP1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RANGAP1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(RANGAP1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RANGAP1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that associates with the nuclear pore complex and participates in the regulation of nuclear transport. The encoded protein interacts with Ras-related nuclear protein 1 (RAN) and regulates guanosine triphosphate (GTP)-binding and exchange. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Uniprot Description
Function: GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state.
Subunit structure: Homodimer. Forms a tight complex in association with RANBP2/NUP358 and UBE2I/UBC9, the ubiquitin-conjugating enzyme E2. Interacts with UBE2I; the interaction conjugates SUMO1 to RANGAP1, and subsequently stabilizes interactions of sumoylated RANGAP1 with RANBP2/NUP358. The SUMO1/RANGAP1/UBC9/NUP358 complex associates with nuclear pore complexes. Interacts with TRAF6. Ref.9 Ref.11
Subcellular location: Cytoplasm. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Chromosome › centromere › kinetochore
By similarity. Cytoplasm › cytoskeleton › spindle pole
By similarity. Note: Cytoplasmic during interphase. Targeted to the nuclear rim after sumoylation. During mitosis, associates with mitotic spindles. Association with kinetochores appears soon after nuclear envelope breakdown and persists until late anaphase. Mitotic location also requires sumoylation. Ref.8 Ref.9
Tissue specificity: Highly expressed in brain, thymus and testis. Ref.7
Post-translational modification: Phosphorylated occurs before nuclear envelope breakdown and continues throughout mitosis. Phosphorylated by the M-phase kinase cyclin B/Cdk1, in vitro. Differential timimg of dephosphorylation occurs during phases of mitosis. The phosphorylated form remains associated with RANBP2/NUP358 and the SUMO E2-conjugating enzyme, UBC9, on nuclear pore complex (NPC) diassembly and during mitosis. Ref.9Sumoylated with SUMO1. Sumoylation is necessary for targeting to the nuclear envelope (NE), and for association with mitotic spindles and kinetochores during mitosis. Also required for interaction with RANBP2 and is mediated by UBC9. Ref.8 Ref.16
Sequence similarities: Belongs to the RNA1 family.Contains 6 LRR (leucine-rich) repeats.
Sequence caution: The sequence BAB47464.1 differs from that shown. Reason: Erroneous initiation.
Research Articles on RANGAP1
Similar Products
Product Notes
The RANGAP1 rangap1 (Catalog #AAA649436) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RANGAP1, NT (RANGAP1, KIAA1835, SD, Ran GTPase-activating protein 1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RANGAP1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot, Immunohistochemistry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50. Researchers should empirically determine the suitability of the RANGAP1 rangap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RANGAP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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