Rabbit anti-Human RAET1E Polyclonal Antibody | anti-RAET1E antibody

RAET1E, ID (RAET1E, LETAL, N2DL4, ULBP4, NKG2D ligand 4, Lymphocyte effector toxicity activation ligand, RAE-1-like transcript 4, Retinoic acid early transcript 1E) (Biotin)

Cat. Num. AAA6340088

• Gene Names: RAET1E; RL-4; LETAL; ULBP4; N2DL-4; NKG2DL4; RAET1E2; bA350J20.7
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RAET1E; Polyclonal Antibody; ID (RAET1E; LETAL; N2DL4; ULBP4; NKG2D ligand 4; Lymphocyte effector toxicity activation ligand; RAE-1-like transcript 4; Retinoic acid early transcript 1E) (Biotin); Retinoic acid early transcript 1E; anti-RAET1E antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-RAET1E antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RAET1E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 150-179 amino acids from the Central region of human RAET1E.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RAET1E Antibody (Center) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the RAET1E antibody detected the RAET1E protein (arrow).)

Related Product Information for anti-RAET1E antibody

Members of the RAET1 family, such as RAET1E, are major histocompatibility complex (MHC) class I-related genes located within a 180-kb cluster on chromosome 6q24.2-q25.3. RAET1 proteins contain MHC class I-like alpha-1 and alpha-2 domains. RAET1E and RAET1G (MIM 609244) differ from the other RAET1 proteins (e.g., RAET1I, or ULBP1; MIM 605697) in that they have type I membrane-spanning sequences at their C termini rather than glycosylphosphatidylinositol anchor sequences. RAET1E is a ligand for NKG2D (KLRK1; MIM 611817), which is expressed on the surface of several types of immune cells and is involved in innate and adaptive immune responses (summary by Radosavljevic et al. (2002) [PubMed 11827464] and Cao et al. (2007) [PubMed 17470428]).

Product Categories/Family for anti-RAET1E antibody

Antibodies; Proteins

References

McGilvray, R.W., et al. Int. J. Cancer 127(6):1412-1420(2010). Antoun, A., et al. Hum. Immunol. 71(6):610-620(2010). Davila, S., et al. Genes Immun. 11(3):232-238(2010). Romphruk, A.V., et al. Immunogenetics 61(9):611-617(2009). Kong, Y., et al. Blood 114(2):310-317(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 21040249
• NCBI GeneID: 135250
• NCBI Accession #: NP_631904.1
• NCBI GenBank Nucleotide #: NM_139165.2
• UniProt Accession #: Q8TD07; Q5VYB7; Q5VYB8; Q8TEZ2; Q96L41; A6YF59
• Molecular Weight: 32,300 Da
• NCBI Official Full Name: retinoic acid early transcript 1E isoform 1
• NCBI Official Synonym Full Names: retinoic acid early transcript 1E
• NCBI Official Symbol: RAET1E
• NCBI Official Synonym Symbols: RL-4; LETAL; ULBP4; N2DL-4; NKG2DL4; RAET1E2; bA350J20.7
• NCBI Protein Information: retinoic acid early transcript 1E
• UniProt Protein Name: NKG2D ligand 4
• Protein Family: Retinoic acid early-inducible protein
• UniProt Gene Name: RAET1E
• UniProt Synonym Gene Names: LETAL; N2DL4; ULBP4; N2DL-4; NKG2DL4; RL-4
• UniProt Entry Name: N2DL4_HUMAN

NCBI Description

This gene belong to the RAET1 family, which consists of major histocompatibility complex (MHC) class I-related genes located in a cluster on chromosome 6q24.2-q25.3. This and RAET1G protein differ from other RAET1 proteins in that they have type I membrane-spanning sequences at their C termini rather than glycosylphosphatidylinositol anchor sequences. This protein functions as a ligand for NKG2D receptor, which is expressed on the surface of several types of immune cells, and is involved in innate and adaptive immune responses. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]

Uniprot Description

RAET1E: Ligand for the NKG2D receptor. Delivers activating signals to NK cells and promotes tumor immune surveillance by inducing the expansion of anti-tumor cytotoxic lymphocytes. Belongs to the MHC class I family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6q25.1

Cellular Component: plasma membrane

Molecular Function: antigen binding; natural killer cell lectin-like receptor binding

Biological Process: antigen processing and presentation; natural killer cell mediated cytotoxicity; regulation of immune response; T cell mediated cytotoxicity