Rabbit anti-Human, Mouse RAET1E Polyclonal Antibody | anti-RAET1E antibody

RAET1E Antibody

Cat. Num. AAA154386

• Gene Names: RAET1E; RL-4; LETAL; ULBP4; N2DL-4; NKG2DL4; RAET1E2; bA350J20.7
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity chromatography purified via peptide column.
• Synonyms: RAET1E; Polyclonal Antibody; RAET1E Antibody; Retinoic acid early transcript 1E; NKG2D ligand 4; N2DL-4; NKG2DL42; RL-4; ULBP4; LETAL; anti-RAET1E antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RAET1E antibody is human and mouse reactive. At least four isoforms of RAET1E are known to exist; this antibody will detect all four.
• Purity/Purification: Affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 263

• Applicable Applications for anti-RAET1E antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: RAET1E antibody can be used for detection of RAET1E by Western blot at 1 - 2 mug/ml.
• Immunogen: RAET1E antibody was raised against an 18 amino acid peptide near the center of human RAET1E.
• Buffer: Supplied in PBS containing 0.02% sodium azide.
• Positive Control 1: EL4 Cell Lysate
• Conjugate: Unconjugated
• Preparation and Storage: Store at 4 degree C for three months and -20 degree C, stable for up to one year.

Western Blot (WB)

(Western blot analysis of RAET1E in EL4 cell lysate with RAET1E antibody at 1 μg/ml.)

Immunocytochemistry (IHC)

(Immunocytochemistry of RAET1E in K562 cells with RAET1E at 2.5 μg/mL. )

Related Product Information for anti-RAET1E antibody

The Retinoic acid early transcript 1E (RAET1E) belongs to the RAET1 family, which consists of major histocompatibility complex (MHC) class I-related genes located in a cluster on chromosome 6q24.2-q25.3 (1). Like the related protein RAET1G, RAET1E differs from other RAET1 proteins in that they have type I membrane-spanning sequences at their carboxy termini rather than glycosylphosphatidylinositol anchor sequences (2). RAET1E functions as a ligand for NKG2D receptor, which is expressed on the surface of several types of immune cells, and is involved in innate and adaptive immune responses (1,3).

Product Categories/Family for anti-RAET1E antibody

Innate Immunity; Cancer

References

Radosavlijevic M, Cuillerier B, Wilson MJ, et al. A cluster of ten novel MHC class I related genes on human chromosome 6q24.2-q25.3. Genomics 2002; 79:114-23. Bacon L, Eagle RA, Meyer M, et al. Two human ULBP/RAET1 molecules with transmembrane regions are ligands for NKG2D. J. Immunol. 2004; 173:1078-84. Letal, a tumor-associated NKG2D immunoreceptor ligand, induces activation and expansion of effector immune cells. Cancer Biol. Ther. 2003; 2:446-51.

NCBI and Uniprot Product Information

• NCBI GI #: 21040249
• NCBI GeneID: 135250
• NCBI Accession #: NP_631904
• NCBI GenBank Nucleotide #: NM_139165.2
• UniProt Accession #: P84103; Q5VYB7; Q5VYB8; Q8TEZ2; Q96L41; A6YF59; Q8TD07
• Molecular Weight: Predicted: 29 kDa; Observed: 29 kDa
• NCBI Official Full Name: NKG2D ligand 4 isoform 1
• NCBI Official Synonym Full Names: retinoic acid early transcript 1E
• NCBI Official Symbol: RAET1E
• NCBI Official Synonym Symbols: RL-4; LETAL; ULBP4; N2DL-4; NKG2DL4; RAET1E2; bA350J20.7
• NCBI Protein Information: NKG2D ligand 4
• UniProt Protein Name: NKG2D ligand 4
• Protein Family: Retinoic acid early-inducible protein
• UniProt Gene Name: RAET1E
• UniProt Synonym Gene Names: LETAL; N2DL4; ULBP4; N2DL-4; NKG2DL4; RL-4
• UniProt Entry Name: N2DL4_HUMAN

NCBI Description

This gene belong to the RAET1 family, which consists of major histocompatibility complex (MHC) class I-related genes located in a cluster on chromosome 6q24.2-q25.3. This and RAET1G protein differ from other RAET1 proteins in that they have type I membrane-spanning sequences at their C termini rather than glycosylphosphatidylinositol anchor sequences. This protein functions as a ligand for NKG2D receptor, which is expressed on the surface of several types of immune cells, and is involved in innate and adaptive immune responses. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]

Uniprot Description

RAET1E: Ligand for the NKG2D receptor. Delivers activating signals to NK cells and promotes tumor immune surveillance by inducing the expansion of anti-tumor cytotoxic lymphocytes. Belongs to the MHC class I family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6q25.1

Cellular Component: extracellular region; integral to membrane; plasma membrane

Molecular Function: antigen binding; natural killer cell lectin-like receptor binding

Biological Process: antigen processing and presentation; natural killer cell mediated cytotoxicity; regulation of immune response; T cell mediated cytotoxicity