Rabbit anti-Human PTPN7 Polyclonal Antibody | anti-PTPN7 antibody

PTPN7 (S44) (Tyrosine-protein Phosphatase Non-receptor Type 7, Protein-tyrosine Phosphatase LC-PTP, Hematopoietic Protein-tyrosine Phosphatase, HEPTP) (AP)

Cat. Num. AAA6495741

• Gene Names: PTPN7; LPTP; HEPTP; PTPNI; BPTP-4; LC-PTP
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: PTPN7; Polyclonal Antibody; PTPN7 (S44) (Tyrosine-protein Phosphatase Non-receptor Type 7; Protein-tyrosine Phosphatase LC-PTP; Hematopoietic Protein-tyrosine Phosphatase; HEPTP) (AP); anti-PTPN7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PTPN7.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 465

• Applicable Applications for anti-PTPN7 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; ELISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to amino acid residues surrounding S44 of human PTPN7 (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-PTPN7 Antibody (S44) in Jurkat cell line lysates (35ug/lane).PTPN7 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PTPN7 Antibody (S44), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-PTPN7 antibody

Antibodies; Enzymes; Phosphatase

References

1. Eswaran, J., Biochem. J. 395(3), 483-491 (2006). 2. Mustelin, T., J. Mol. Biol. 354(1), 150-163 (2005). 3. Pettiford, S.M., Leukemia 17(2), 366-378 (2003). 4. Kosaki, K., J. Clin. Endocrinol. Metab. 87(8), 3529-3533 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 315221140
• NCBI GeneID: 5778
• NCBI Accession #: NP_002823
• NCBI GenBank Nucleotide #: NP_002823.3
• UniProt Accession #: P35236
• Molecular Weight: 40.398
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type 7 isoform 1
• NCBI Official Synonym Full Names: protein tyrosine phosphatase non-receptor type 7
• NCBI Official Symbol: PTPN7
• NCBI Official Synonym Symbols: LPTP; HEPTP; PTPNI; BPTP-4; LC-PTP
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type 7
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type 7
• Protein Family: Tyrosine-protein phosphatase non-receptor
• UniProt Gene Name: PTPN7
• UniProt Synonym Gene Names: HEPTP
• UniProt Entry Name: PTN7_HUMAN

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This gene is preferentially expressed in a variety of hematopoietic cells, and is an early response gene in lymphokine stimulated cells. The non-catalytic N-terminus of this PTP can interact with MAP kinases and suppress the MAP kinase activities. This PTP was shown to be involved in the regulation of T cell antigen receptor (TCR) signaling, which was thought to function through dephosphorylating the molecules related to MAP kinase pathway. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

HePTP: a non-receptor protein-tyrosine phosphatase. Preferentially expressed in a variety of hematopoietic cells, and is an early response gene in lymphokine stimulated cells. Its noncatalytic N-terminus can interact with MAP kinases and suppress the MAP kinase activities. Shown to be involved in the regulation of T cell antigen receptor (TCR) signaling, which was thought to function through dephosphorylating the molecules related to MAP kinase pathway. Three alternatively spliced transcript variants of this gene, which encode two distinct isoforms, are reported. May play a role in the regulation of lymphocyte development.

Protein type: Motility/polarity/chemotaxis; EC 3.1.3.48; Protein phosphatase, tyrosine (non-receptor)

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: internal side of plasma membrane; cytoskeleton; cytoplasm; cytosol

Molecular Function: protein binding; protein tyrosine phosphatase activity

Biological Process: protein amino acid dephosphorylation