Rabbit anti-Mouse DCC Polyclonal Antibody | anti-DCC antibody

DCC, ID (DCC, IGDCC1, Netrin receptor DCC, Colorectal cancer suppressor, Immunoglobulin superfamily DCC subclass member 1, Tumor suppressor protein DCC) (PE)

Cat. Num. AAA6291023

• Gene Names: DCC; CRC18; CRCR1; MRMV1; IGDCC1
• Reactivity: Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: DCC; Polyclonal Antibody; ID (DCC; IGDCC1; Netrin receptor DCC; Colorectal cancer suppressor; Immunoglobulin superfamily DCC subclass member 1; Tumor suppressor protein DCC) (PE); Tumor suppressor protein DCC; anti-DCC antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse DCC.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-DCC antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 643-670 in the central region of mouse DCC, UniProt Accession #NP_005206.2; P43146; Q63155; P70211. Species sequence homology: rat
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse heart tissue lysates (35ug/lane) usingMBS6000986. This demonstrates that MBS6000986 detected the DCC protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue using MBS6000986 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6000986 for immunohistochemistry.)

Related Product Information for anti-DCC antibody

This gene encodes a netrin 1 receptor. The transmembrane protein is a member of the immunoglobulin superfamily of cell adhesion molecules, and mediates axon guidance of neuronal growth cones towards sources of netrin 1 ligand. The cytoplasmic tail interacts with the tyrosine kinases Src and focal adhesion kinase (FAK, also known as PTK2) to mediate axon attraction. The protein partially localizes to lipid rafts, and induces apoptosis in the absence of ligand. The protein functions as a tumor suppressor, and is frequently mutated or downregulated in colorectal cancer and esophageal carcinoma.

Product Categories/Family for anti-DCC antibody

Antibodies; Netrin

References

1. Shimada, M., et al. Hum. Genet. 128(4):433-441(2010). 2. Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010). 3. Srour, M., et al. Science 328 (5978), 592 (2010). 4. Docherty, S.J., et al. BMC Genet. 11, 61 (2010). 5. Li, W., et al. Nat. Neurosci. 7(11):1213-1221(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 110431348
• NCBI GeneID: 1630
• NCBI Accession #: NP_005206.2
• NCBI GenBank Nucleotide #: NM_005215.3
• UniProt Accession #: P43146; Q63155; P70211
• Molecular Weight: 158,457 Da
• NCBI Official Full Name: netrin receptor DCC
• NCBI Official Synonym Full Names: deleted in colorectal carcinoma
• NCBI Official Symbol: DCC
• NCBI Official Synonym Symbols: CRC18; CRCR1; MRMV1; IGDCC1
• NCBI Protein Information: netrin receptor DCC; colorectal tumor suppressor; colorectal cancer suppressor; tumor suppressor protein DCC; deleted in colorectal cancer protein; immunoglobulin superfamily DCC subclass member 1; immunoglobulin superfamily, DCC subclass, member 1
• UniProt Protein Name: Netrin receptor DCC
• Protein Family: DCC family protein
• UniProt Gene Name: DCC
• UniProt Synonym Gene Names: IGDCC1
• UniProt Entry Name: DCC_HUMAN

NCBI Description

This gene encodes a netrin 1 receptor. The transmembrane protein is a member of the immunoglobulin superfamily of cell adhesion molecules, and mediates axon guidance of neuronal growth cones towards sources of netrin 1 ligand. The cytoplasmic tail interacts with the tyrosine kinases Src and focal adhesion kinase (FAK, also known as PTK2) to mediate axon attraction. The protein partially localizes to lipid rafts, and induces apoptosis in the absence of ligand. The protein functions as a tumor suppressor, and is frequently mutated or downregulated in colorectal cancer and esophageal carcinoma. [provided by RefSeq, Oct 2009]

Uniprot Description

DCC: Receptor for netrin required for axon guidance. Mediates axon attraction of neuronal growth cones in the developing nervous system upon ligand binding. Its association with UNC5 proteins may trigger signaling for axon repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Implicated as a tumor suppressor gene. Defects in DCC are the cause of mirror movements type 1 (MRMV1). A disorder characterized by contralateral involuntary movements that mirror voluntary ones. While mirror movements are occasionally found in young children, persistence beyond the age of 10 is abnormal. Mirror movements occur more commonly in the upper extremities. Belongs to the immunoglobulin superfamily. DCC family.

Protein type: Membrane protein, integral; Immunoglobulin superfamily; Tumor suppressor; Receptor, misc.

Chromosomal Location of Human Ortholog: 18q21.3

Cellular Component: axon; integral to membrane; plasma membrane; cytosol; lipid raft

Molecular Function: identical protein binding; protein binding; transmembrane receptor activity; netrin receptor activity; transcription coactivator activity

Biological Process: axon guidance; axonogenesis; spinal cord ventral commissure morphogenesis; positive regulation of apoptosis; apoptosis; response to amphetamine; neuron migration; negative regulation of collateral sprouting; dorsal/ventral axon guidance; anterior/posterior axon guidance

Disease: Mirror Movements 1; Esophageal Cancer; Colorectal Cancer