Rabbit anti-Human PTPN20A Polyclonal Antibody | anti-PTPN20A antibody

PTPN20A Antibody (Center)

Cat. Num. AAA9206867

• Gene Names: PTPN20; CT126; PTPN20A; PTPN20B; hPTPN20; bA42B19.1; bA142I17.1
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: PTPN20A; Polyclonal Antibody; PTPN20A Antibody (Center); Tyrosine-protein phosphatase non-receptor type 20; hPTPN20; anti-PTPN20A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This PTPN20A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178-207 amino acids from the Central region of human PTPN20A.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.32 (varies by lot)
• Sequence Positions: 178-207
• Sequence Length: 420

• Applicable Applications for anti-PTPN20A antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(PTPN20A Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PTPN20A antibody detected the PTPN20A protein (arrow).)

Immunohistochemistry (IHC)

(PTPN20A Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PTPN20A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(PTPN20A Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-PTPN20A antibody

The product of this gene belongs to the family of
classical tyrosine-specific protein tyrosine phosphatases. Many
protein tyrosine phosphatases have been shown to regulate
fundamental cellular processes and several are mutated in human
diseases. Chromosome 10q contains a segmental duplication resulting
in multiple copies of the protein tyrosine phosphatase,
non-receptor type 20 gene. The two nearly identical copies are
designated as PTPN20A and PTPN20B. A third copy is only partially
duplicated and contains a pseudogene, designated as PTPN20C. This
gene encodes the more telomeric copy, PTPN20B. Multiple
alternatively spliced transcript variants encoding different
isoforms have been identified.

Product Categories/Family for anti-PTPN20A antibody

Signal Transduction

References

Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
Fodero-Tavoletti, M.T., et al. Biochem. J. 389 (PT 2), 343-354 (2005) :

NCBI and Uniprot Product Information

• NCBI GI #: 108802604
• NCBI GeneID: 26095
• NCBI Accession #: NP_001035816.1; NP_001035817.1; NP_001035818.1; NP_001035819.1; NP_001035820.1; NP_001035821.1; NP_001035822.1; NP_001035823.1; NP_001035846.1; NP_001035848.1; NP_001035849.1; NP_001035850.1; NP_001035851.1; NP_00
• NCBI GenBank Nucleotide #: NM_001042357.3
• UniProt Accession #: Q4JDL3; Q4JDG6; Q4JDK1; Q4JDK5; Q4JDK6; Q4JDK8; Q4JDK9; Q4JDL0; Q4JDL1; Q4JDL4; A6NNH8; B7ZKV3
• Molecular Weight: 48423
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type 20 isoform 1
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, non-receptor type 20
• NCBI Official Symbol: PTPN20
• NCBI Official Synonym Symbols: CT126; PTPN20A; PTPN20B; hPTPN20; bA42B19.1; bA142I17.1
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type 20
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type 20
• UniProt Gene Name: PTPN20
• UniProt Synonym Gene Names: hPTPN20
• UniProt Entry Name: PTN20_HUMAN

NCBI Description

The product of this gene belongs to the family of classical tyrosine-specific protein tyrosine phosphatases. Many protein tyrosine phosphatases have been shown to regulate fundamental cellular processes. The encoded protein appears to be targeted to sites of actin polymerization. A pseudogene of this gene has been defined on chromosome 10. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]

Uniprot Description

PTPN20A: Tyrosine-protein phosphatase targeted to sites of actin polymerization in response of varied extracellular stimuli. Has tyrosine phosphatase activity towards various tyrosyl phosphorylated substrates. Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily. 15 isoforms of the human protein are produced by alternative splicing.

Protein type: Cancer Testis Antigen (CTA); Protein phosphatase, tyrosine (non-receptor); EC 3.1.3.48

Chromosomal Location of Human Ortholog: 10q11.22

Cellular Component: microtubule; cytoplasm; microtubule organizing center; nucleus

Molecular Function: protein tyrosine phosphatase activity