Rabbit anti-Human PTPN20A Polyclonal Antibody | anti-PTPN20A antibody

PTPN20A, ID (PTPN20A, Tyrosine-protein phosphatase non-receptor type 20) (FITC)

Cat. Num. AAA6338989

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PTPN20A; Polyclonal Antibody; ID (PTPN20A; Tyrosine-protein phosphatase non-receptor type 20) (FITC); Tyrosine-protein phosphatase non-receptor type 20; anti-PTPN20A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 411

• Applicable Applications for anti-PTPN20A antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PTPN20A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 185-215 amino acids from the Central region of human PTPN20A.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PTPN20A Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PTPN20A antibody detected the PTPN20A protein (arrow).)

Immunohistochemistry (IHC)

(PTPN20A Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PTPN20A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(PTPN20A Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-PTPN20A antibody

The product of this gene belongs to the family of classical tyrosine-specific protein tyrosine phosphatases. Many protein tyrosine phosphatases have been shown to regulate fundamental cellular processes and several are mutated in human diseases. Chromosome 10q contains a segmental duplication resulting in multiple copies of the protein tyrosine phosphatase, non-receptor type 20 gene. The two nearly identical copies are designated as PTPN20A and PTPN20B. A third copy is only partially duplicated and contains a pseudogene, designated as PTPN20C. This gene encodes the more telomeric copy, PTPN20B. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.

Product Categories/Family for anti-PTPN20A antibody

Antibodies; Enzymes; Phosphatase

References

Matsuoka, S., et al. Science 316(5828):1160-1166(2007). Fodero-Tavoletti, M.T., et al. Biochem. J. 389 (PT 2), 343-354 (2005).

NCBI and Uniprot Product Information

• NCBI GI #: 108802607
• NCBI GeneID: 26095; 653129
• NCBI Accession #: NP_001035817.1; NP_001035853.1; NP_001035849.1
• NCBI GenBank Nucleotide #: NP_001035817.1
• UniProt Accession #: Q4JDL3
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type 20 isoform 3
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type 20
• UniProt Gene Name: PTPN20
• UniProt Synonym Gene Names: hPTPN20

Uniprot Description

Tyrosine-protein phosphatase targeted to sites of actin polymerization in response of varied extracellular stimuli. Has tyrosine phosphatase activity towards various tyrosyl phosphorylated substrates.