Rabbit PTEN Polyclonal Antibody | anti-PTEN antibody

PTEN(Phospho-Ser370) Antibody

Cat. Num. AAA9404599

• Gene Names: PTEN; BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: PTEN; Polyclonal Antibody; PTEN(Phospho-Ser370) Antibody; BZS; DEC; GLM2; MHAM; TEP1; anti-PTEN antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of PTEN only when phosphorylated at serine 370.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 403

• Applicable Applications for anti-PTEN antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500; Immunohistochemistry: 1:50~1:100; Immunofluorescence: 1:100~1:200
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of serine 370(D-V-S(p)-D-N) derived from Human PTEN.
• Modification: Phospho-Ser370
• Target Name: PTEN
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells using PTEN (phospho-Ser370) antibody ().)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using PTEN (phospho-Ser370) antibody ().)

Immunofluorescence (IF)

(Immunofluorescence staining of methanol-fixed MCF7 cells using PTEN (phospho-Ser370) antibody (, Red).)

Related Product Information for anti-PTEN antibody

Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form induces less tumor suppressive ability. 

Product Categories/Family for anti-PTEN antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 73765544
• NCBI GeneID: 5728
• NCBI Accession #: NP_000305.3
• NCBI GenBank Nucleotide #: NM_000314.6
• UniProt Accession #: P60484; O00633; O02679; Q6ICT7; B2R904; F2YHV0
• Molecular Weight: 19,796 Da
• NCBI Official Full Name: phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN isoform PTEN
• NCBI Official Synonym Full Names: phosphatase and tensin homolog
• NCBI Official Symbol: PTEN
• NCBI Official Synonym Symbols: BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del
• NCBI Protein Information: phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
• UniProt Protein Name: Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
• Protein Family: Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase
• UniProt Gene Name: PTEN
• UniProt Synonym Gene Names: MMAC1; TEP1
• UniProt Entry Name: PTEN_HUMAN

NCBI Description

This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2015]

Uniprot Description

PTEN: a phosphoinositide 3-phosphatase (PIP3) and a tumor suppressor implicated in a wide variety of human cancers. Dephosphorylates inositol phospholipids generated by the activation of the phosphoinositide 3 kinase (PI3K). A major negative regulator of the PI3K/Akt signaling pathway. Possesses a C-terminal regulatory domain that contains three phosphorylation sites which may regulate its stability.

Protein type: EC 3.1.3.16; EC 3.1.3.48; Phosphatase, lipid; EC 3.1.3.67; Carbohydrate Metabolism - inositol phosphate; Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Tumor suppressor

Chromosomal Location of Human Ortholog: 10q23.3

Cellular Component: nucleoplasm; postsynaptic membrane; PML body; internal side of plasma membrane; neuron projection; cell projection; mitochondrion; cytoplasm; plasma membrane; dendritic spine; extracellular region; nucleus; cytosol

Molecular Function: protein binding; phosphatidylinositol-3-phosphatase activity; enzyme binding; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity; phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity; protein tyrosine/serine/threonine phosphatase activity; inositol-1,3,4,5-tetrakisphosphate 3-phosphatase activity; magnesium ion binding; platelet-derived growth factor receptor binding; protein tyrosine phosphatase activity; protein serine/threonine phosphatase activity; phosphoprotein phosphatase activity; lipid binding; protein kinase binding; PDZ domain binding

Biological Process: male mating behavior; central nervous system development; nerve growth factor receptor signaling pathway; prepulse inhibition; regulation of cellular component size; response to arsenic; heart development; anaphase-promoting complex activation during mitotic cell cycle; Wnt receptor signaling pathway through beta-catenin; T cell receptor signaling pathway; regulation of B cell apoptosis; myelin maintenance in the central nervous system; response to glucose stimulus; negative regulation of axonogenesis; response to drug; rhythmic synaptic transmission; dentate gyrus development; fibroblast growth factor receptor signaling pathway; negative regulation of myelination; social behavior; forebrain morphogenesis; negative regulation of organ growth; response to ethanol; response to zinc ion; inositol phosphate dephosphorylation; phosphatidylinositol biosynthetic process; endothelial cell migration; positive regulation of transcription factor activity; multicellular organismal response to stress; negative regulation of apoptosis; negative regulation of phagocytosis; synapse maturation; inositol phosphate metabolic process; apoptosis; regulation of protein stability; platelet-derived growth factor receptor signaling pathway; locomotory behavior; negative regulation of cell size; protein amino acid dephosphorylation; regulation of myeloid cell apoptosis; response to estradiol stimulus; locomotor rhythm; negative regulation of cell proliferation; synaptogenesis; learning and/or memory; negative regulation of protein amino acid phosphorylation; phospholipid metabolic process; positive regulation of cell proliferation; protein kinase B signaling cascade; brain morphogenesis; nerve-nerve synaptic transmission; angiogenesis; negative regulation of cell migration; response to nutrient; aging; negative regulation of epithelial cell proliferation; epidermal growth factor receptor signaling pathway; cardiac muscle development; cell migration; phosphoinositide-mediated signaling; negative regulation of protein kinase B signaling cascade; central nervous system neuron axonogenesis; protein stabilization; maternal behavior; negative regulation of focal adhesion formation; response to ATP; memory; cell proliferation; G1/S-specific negative regulation of cyclin-dependent protein kinase activity; innate immune response; phosphoinositide dephosphorylation; regulation of cyclin-dependent protein kinase activity; negative regulation of phosphoinositide 3-kinase cascade

Disease: Meningioma, Familial, Susceptibility To; Thyroid Carcinoma, Follicular; Melanoma, Cutaneous Malignant, Susceptibility To, 1; Bannayan-riley-ruvalcaba Syndrome; Squamous Cell Carcinoma, Head And Neck; Cowden Syndrome 1; Glioma Susceptibility 2; Prostate Cancer; Endometrial Cancer; Vacterl Association With Hydrocephalus; Macrocephaly/autism Syndrome