Rabbit PTEN Polyclonal Antibody | anti-PTEN antibody

PTEN (Ab-380/382/383) Antibody

Cat. Num. AAA9411835

• Gene Names: PTEN; BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• Synonyms: PTEN; Polyclonal Antibody; PTEN (Ab-380/382/383) Antibody; MMAC1; Mutated in multiple advanced cancers 1; Protein-tyrosine phosphatase PTEN; TEP1; anti-PTEN antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total PTEN protein.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 403

• Applicable Applications for anti-PTEN antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500~1:1000; Immunohistochemistry: 1:50~1:100
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around aa.378~382/380~384/381~385 (R-Y-S-D-T-T-D-S) derived from Human PTEN.
• Target Name: PTEN
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Western Blot (WB)

(Western blot analysis of extracts from HT29 cells using PTEN(Ab-380/382/383) Antibody and the same antibody preincubated with blocking peptide.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PTEN(Ab-380/382/383) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

Related Product Information for anti-PTEN antibody

Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue.

Product Categories/Family for anti-PTEN antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 73765544
• NCBI GeneID: 5728
• NCBI Accession #: NP_000305.3
• NCBI GenBank Nucleotide #: NM_000314.6
• UniProt Accession #: P60484; O00633; O02679; Q6ICT7; B2R904; F2YHV0
• Molecular Weight: 19,796 Da
• NCBI Official Full Name: phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN isoform PTEN
• NCBI Official Synonym Full Names: phosphatase and tensin homolog
• NCBI Official Symbol: PTEN
• NCBI Official Synonym Symbols: BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del
• NCBI Protein Information: phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
• UniProt Protein Name: Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
• Protein Family: Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase
• UniProt Gene Name: PTEN
• UniProt Synonym Gene Names: MMAC1; TEP1
• UniProt Entry Name: PTEN_HUMAN

NCBI Description

This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2015]

Uniprot Description

PTEN: a phosphoinositide 3-phosphatase (PIP3) and a tumor suppressor implicated in a wide variety of human cancers. Dephosphorylates inositol phospholipids generated by the activation of the phosphoinositide 3 kinase (PI3K). A major negative regulator of the PI3K/Akt signaling pathway. Possesses a C-terminal regulatory domain that contains three phosphorylation sites which may regulate its stability.

Protein type: Carbohydrate Metabolism - inositol phosphate; Phosphatase, lipid; Nuclear receptor co-regulator; Tumor suppressor; EC 3.1.3.67; Motility/polarity/chemotaxis; EC 3.1.3.16; EC 3.1.3.48

Chromosomal Location of Human Ortholog: 10q23.3

Cellular Component: PML body; neuron projection; internal side of plasma membrane; mitochondrion; dendritic spine; extracellular region; cytosol; nucleoplasm; postsynaptic membrane; cell projection; cytoplasm; plasma membrane; nucleus

Molecular Function: phosphatidylinositol-3-phosphatase activity; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity; inositol-1,3,4,5-tetrakisphosphate 3-phosphatase activity; platelet-derived growth factor receptor binding; magnesium ion binding; protein tyrosine phosphatase activity; protein serine/threonine phosphatase activity; protein kinase binding; PDZ domain binding; protein binding; enzyme binding; phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity; protein tyrosine/serine/threonine phosphatase activity; lipid binding; phosphoprotein phosphatase activity

Biological Process: central nervous system development; male mating behavior; prepulse inhibition; nerve growth factor receptor signaling pathway; response to arsenic; heart development; regulation of cellular component size; anaphase-promoting complex activation during mitotic cell cycle; Wnt receptor signaling pathway through beta-catenin; T cell receptor signaling pathway; regulation of B cell apoptosis; myelin maintenance in the central nervous system; response to glucose stimulus; negative regulation of axonogenesis; response to drug; rhythmic synaptic transmission; dentate gyrus development; fibroblast growth factor receptor signaling pathway; negative regulation of myelination; social behavior; negative regulation of organ growth; forebrain morphogenesis; response to ethanol; response to zinc ion; inositol phosphate dephosphorylation; phosphatidylinositol biosynthetic process; endothelial cell migration; positive regulation of transcription factor activity; multicellular organismal response to stress; negative regulation of apoptosis; negative regulation of phagocytosis; synapse maturation; inositol phosphate metabolic process; apoptosis; regulation of protein stability; negative regulation of cell size; locomotory behavior; platelet-derived growth factor receptor signaling pathway; regulation of myeloid cell apoptosis; protein amino acid dephosphorylation; response to estradiol stimulus; locomotor rhythm; negative regulation of cell proliferation; synaptogenesis; learning and/or memory; negative regulation of protein amino acid phosphorylation; phospholipid metabolic process; positive regulation of cell proliferation; brain morphogenesis; protein kinase B signaling cascade; nerve-nerve synaptic transmission; angiogenesis; negative regulation of cell migration; response to nutrient; negative regulation of epithelial cell proliferation; aging; cardiac muscle development; epidermal growth factor receptor signaling pathway; cell migration; negative regulation of protein kinase B signaling cascade; phosphoinositide-mediated signaling; protein stabilization; central nervous system neuron axonogenesis; maternal behavior; negative regulation of focal adhesion formation; response to ATP; memory; cell proliferation; G1/S-specific negative regulation of cyclin-dependent protein kinase activity; phosphoinositide dephosphorylation; innate immune response; regulation of cyclin-dependent protein kinase activity; negative regulation of phosphoinositide 3-kinase cascade

Disease: Meningioma, Familial, Susceptibility To; Melanoma, Cutaneous Malignant, Susceptibility To, 1; Thyroid Carcinoma, Follicular; Bannayan-riley-ruvalcaba Syndrome; Cowden Syndrome 1; Squamous Cell Carcinoma, Head And Neck; Prostate Cancer; Glioma Susceptibility 2; Endometrial Cancer; Vacterl Association With Hydrocephalus; Macrocephaly/autism Syndrome