Rabbit Eukaryotic Translation Initiation Factor 2B, epsilon Polyclonal Antibody | anti-EIF2B5 antibody

Eukaryotic Translation Initiation Factor 2B, epsilon (eIF2B-e)

Cat. Num. AAA614000

• Gene Names: EIF2B5; CLE; CACH; LVWM; EIF-2B; EIF2Bepsilon
• Reactivity: Human, Monkey, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A and immunoaffinity chromatography.
• Synonyms: Eukaryotic Translation Initiation Factor 2B; epsilon; Polyclonal Antibody; epsilon (eIF2B-e); Anti -Eukaryotic Translation Initiation Factor 2B; anti-EIF2B5 antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human endogenous levels of total eIF2B-e protein. Species Crossreactivity: Mouse, rat and monkey.
• Purity/Purification: Affinity Purified; Purified by Protein A and immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 0.1mg/ml BSA and 50% glycerol.

• Applicable Applications for anti-EIF2B5 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1:1000.
• Immunogen: Synthetic peptide corresponding to amino acids near the middle of human eIF2B-e (KLH). MW: 85kD.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from HT29 cells, using MBS614000 eIF2B-epsilon.)

Immunofluorescence (IF)

(Immunofluorescent analysis of HT29 cells, using MBS614000 eIF2B-epsilon Antibody.)

Related Product Information for anti-EIF2B5 antibody

Phosphorylation of the a subunit of eukaryotic initiation factor 2 is a well documented mechanism of down regulating protein synthesis under a variety of stress conditions. Eukaryotic initiation factor 2 binds GTP and Met-tRNAi and transfers the Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). For eIF2 to promote a new round of translation initiation, GDP must be exchanged for GTP, a reaction catalyzed by eIF2B (1,2). Kinases activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2) and hemin deficiency (HRI) can phosphorylate the a subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2B complex, inhibiting the turnover of eIF2B. Induction of PKR by IFN-g and TNF-a, or stress provoked by depletion of endoplasmic reticulum calcium levels, induces potent phosphorylation of eIF2a at Ser51 (5,6). eIF2B, a guanine nucleotide exchange factor, is composed of 5 subunits, the largest of which is eIF2B-e (7). Multiple in vivo phosphorylation sites have been identified on eIF2B-e (8). Casein Kinase II can phosphorylate eIF2B-e at Ser717/718 to allow for association with its substrate eIF2. Phosphorylation at Ser544 allows GSK-3 to phosphorylate the key regulatory site Ser540. A fifth eIF2B-e phosphorylation site, Ser466, can be phosphorylated by casein kinase I.

Product Categories/Family for anti-EIF2B5 antibody

Antibodies; Abs to Translation Initiation Factors

NCBI and Uniprot Product Information

• NCBI GI #: 119598705
• NCBI GeneID: 8893
• UniProt Accession #: Q13144; Q541Z1; Q96D04; Q59HD8
• Molecular Weight: 80,380 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa, isoform CRA_b
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa
• NCBI Official Symbol: EIF2B5
• NCBI Official Synonym Symbols: CLE; CACH; LVWM; EIF-2B; EIF2Bepsilon
• NCBI Protein Information: translation initiation factor eIF-2B subunit epsilon; OTTHUMP00000210774; OTTHUMP00000210847; eIF-2B GDP-GTP exchange factor subunit epsilon
• UniProt Protein Name: Translation initiation factor eIF-2B subunit epsilon
• Protein Family: Translation initiation factor
• UniProt Gene Name: EIF2B5
• UniProt Synonym Gene Names: EIF2BE
• UniProt Entry Name: EI2BE_HUMAN

NCBI Description

This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter. [provided by RefSeq]

Uniprot Description

eIF2B-epsilon: eukaryotic translation initiation factor 2B, subunit 5. A translational regulatory protein that functions in the early steps of protein synthesis by catalyzing the exchange of eukaryotic initiation factor 2-bound GDP for GTP. Mutation in EIF2B5 causes childhood ataxia with central nervous system hypomyelination/ vanishing white matter leukodystrophy.

Protein type: Translation; Translation initiation

Chromosomal Location of Human Ortholog: 3q27.1

Cellular Component: eukaryotic translation initiation factor 2B complex; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; translation initiation factor binding; guanyl-nucleotide exchange factor activity; translation initiation factor activity

Biological Process: myelination; response to peptide hormone stimulus; translation; astrocyte development; positive regulation of translational initiation; cellular protein metabolic process; astrocyte differentiation; ovarian follicle development; response to heat; translational initiation; response to glucose stimulus; negative regulation of translation initiation in response to stress; gene expression; oligodendrocyte development; positive regulation of GTPase activity

Disease: Leukoencephalopathy With Vanishing White Matter