Western Blot (WB)
(Western blot analysis of Recombinant PP1alpha, incubated with or without cdc2 protein kinase in the presence of ATP and kinase assay buffer at 30°C for 30 minutes, using Phospho-PP1alpha (Thr320) Antibody (upper) or control PP1alpha Antibody #P9107-10A (lower).)
Rabbit Protein Phosphotase 1, alpha Polyclonal Antibody
Protein Phosphotase 1, alpha, phosphorylated (Thr320) (PP1a)
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
Protein Phosphotase 1; alpha; Polyclonal Antibody; phosphorylated (Thr320) (PP1a); Anti -Protein Phosphotase 1; anti-Protein Phosphotase 1; alpha antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Detects human PP1 alpha only when phosphorylated at Thr320. May crossreact with phosphorylated PP1 isoforms delta or gamma.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Applicable Applications for anti-Protein Phosphotase 1, alpha antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blott, and Immunohistochemistry (Paraffin).
Dilution: Western Blot: 1:1000
Immunohistochemistry (Paraffin): 1:50
Dilution: Western Blot: 1:1000
Immunohistochemistry (Paraffin): 1:50
Immunogen
Synthetic phospho-Thr320 peptide (KLH coupled) corresponding to residues surrounding Thr320 of human PP1 alpha.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of Recombinant PP1alpha, incubated with or without cdc2 protein kinase in the presence of ATP and kinase assay buffer at 30°C for 30 minutes, using Phospho-PP1alpha (Thr320) Antibody (upper) or control PP1alpha Antibody #P9107-10A (lower).)
Western Blot (WB)
(Western blot analysis of Recombinant PP1alpha, incubated with or without cdc2 protein kinase in the presence of ATP and kinase assay buffer at 30°C for 30 minutes, using Phospho-PP1alpha (Thr320) Antibody (upper) or control PP1alpha Antibody #P9107-10A (lower).)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, asynchronous (lane 1), G2/M (lane 2) or released from G2/M for 6 hours (lane 3), using Phospho-PP1alpha (Thr320) Antibody (upper) or PP1alpha Antibody #P9107-10A (lower).)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, asynchronous (lane 1), G2/M (lane 2) or released from G2/M for 6 hours (lane 3), using Phospho-PP1alpha (Thr320) Antibody (upper) or PP1alpha Antibody #P9107-10A (lower).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear localization, using Phospho-PP1alpha (Thr320) Antibody.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear localization, using Phospho-PP1alpha (Thr320) Antibody.)
Related Product Information for anti-Protein Phosphotase 1, alpha antibody
Type 1 protein phosphatase (PP1), a serine/threonine phosphatase, is highly conserved in eukaryotic cells. Four isoforms of PP1 have been characterized: PP1 alpha, PP1 delta, PP1 gamma1 and PP1 gamma2. Involvement in the cell cycle regulation is one of the biological functions of PP1. It has been illustrated that PP1 dephosphorylates pRB and cdc25 during mitosis. The activity and specificity of PP1 is highly regulated by regulatory subunits and targeting proteins. In addition, a cell-cycle dependent phosphorylation at threonine 320 of PP1• by cdc2 kinase has been reported recently, which inhibits PP1• activity.
Product Categories/Family for anti-Protein Phosphotase 1, alpha antibody
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Product Notes
The Protein Phosphotase 1, alpha (Catalog #AAA614044) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Protein Phosphotase 1, alpha, phosphorylated (Thr320) (PP1a) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Protein Phosphotase 1, alpha can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blott, and Immunohistochemistry (Paraffin). Dilution: Western Blot: 1:1000 Immunohistochemistry (Paraffin): 1:50. Researchers should empirically determine the suitability of the Protein Phosphotase 1, alpha for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Protein Phosphotase 1, alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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