Rabbit Protein Kinase C, delta, theta Polyclonal Antibody | anti-Pkcdelta antibody

Protein Kinase C, delta, theta, phosphorylated (Ser643/676) (PKCd,t)

Cat. Num. AAA612607

• Gene Names: Pkcdelta; CG10524; CG11245; CG42349; DmelCG42349; PKCd
• Reactivity: Human, Monkey, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Protein Kinase C; delta; theta; Polyclonal Antibody; phosphorylated (Ser643/676) (PKCd; t); Anti -Protein Kinase C; anti-Pkcdelta antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of rat PKC delta only when phosphorylated at serines 643 and
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

• Applicable Applications for anti-Pkcdelta antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Dilution: Western Blotting: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.; Immunohistochemistry (Paraffin): 1:50
• Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser643 of rat PKC delta.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of Baculovirus expressed PKC isoforms alpha, beta, gamma, delta and epsilon, untreated or lambda protein phosphatase-treated, using Phospho-PKCdelta/theta (Ser643/676) Antibody (upper) or PKCalpha, -beta, -gamma, -delta and -epsilon antibodies (lower).)

Western Blot (WB)

(Western blot analysis of extracts from U-937 cells, untreated or TPA-treated (0.2 µM), using Phospho-PKCdelta/theta (Ser643/676) Antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-PKCdelta/theta (Ser643/676) Antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-PKCdelta/theta (Ser643/676). Antibody.)

Testing Data

(Phosphorylation of PKCdelta.)

Related Product Information for anti-Pkcdelta antibody

Activation of PKC is one of the earliest events in the cascade leading to a variety of cellular responses such as secretion, gene expression, proliferation and muscle contraction (1,2). PKC isoforms have been classified in three groups: classical PKCs, which

Product Categories/Family for anti-Pkcdelta antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 85725280
• NCBI GeneID: 32191
• NCBI Accession #: NP_511171.3
• NCBI GenBank Nucleotide #: NM_078616.3
• UniProt Accession #: P83099; Q5BIE9; Q9VYN2
• Molecular Weight: 76,333 Da
• NCBI Official Full Name: protein kinase C delta, isoform C
• NCBI Official Synonym Full Names: Protein kinase C delta
• NCBI Official Symbol: Pkcdelta
• NCBI Official Synonym Symbols: CG10524; CG11245; CG42349; DmelCG42349; PKCd
• NCBI Protein Information: CG42349 gene product from transcript CG42349-RC; protein kinase C delta, isoform C; CG42349-PC; Pkcdelta-PC; protein kinase C delta, isoform D; CG42349-PD; Pkcdelta-PD; protein kinase C delta, isoform B; CG42349-PB; Pkcdelta-PB
• UniProt Protein Name: Putative protein kinase C delta type homolog
• Protein Family: Putative protein kinase
• UniProt Gene Name: Pkcdelta
• UniProt Entry Name: KPC4_DROME

Uniprot Description

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Sequence similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.Contains 1 AGC-kinase C-terminal domain.Contains 2 phorbol-ester/DAG-type zinc fingers.Contains 1 protein kinase domain.