Rabbit anti-Mouse Phospholipase A2, Calcium Independent (iPLA2) Polyclonal Antibody | anti-iPLA2 antibody

Polyclonal Antibody to Phospholipase A2, Calcium Independent (iPLA2)

Cat. Num. AAA2013807

• Gene Names: Pla2g6; iPLA2; PNPLA9; BB112799; iPLA2beta; iPLA(2)beta
• Reactivity: Mouse
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
• Purity: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Synonyms: Phospholipase A2; Calcium Independent (iPLA2); Polyclonal Antibody; Polyclonal Antibody to Phospholipase A2; anti-iPLA2 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Purity/Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
• Concentration: 0.22mg/ml (varies by lot)
• Sequence: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LLCLDGGGVK GLVIIQLLIA IEKASGVATK DLFDWVAGTS TGGILALAIL HSKSMAYMRG VYFRMKDEVF RGSRPYESGP LEEFLKREFG EHTKMTDVKK PKVMLTGTLS DRQPAELHLF RNYDAPEAVR EPRCNQNINL KPPTQPADQL VWRAARSSGA APTYFRPNGR FLDGGLLANN PTLDAMTEIH EYNQDMIRKG

• Applicable Applications for anti-iPLA2 antibody: Western Blot (WB), Immunohistochemistry (IHC) - Formalin/Paraffin, Immunocytochemistry (ICC), Immunoprecipitatio (IP).
• Application Notes: Western blotting: 0.5-2ug/mL; Immunohistochemistry: 5-20ug/mL; Immunocytochemistry: 5-20ug/mL; Optimal working dilutions must be determined by end user.
• Organism Species: Mus musculus (Mouse)
• Source: Polyclonal antibody preparation
• Traits: Liquid
• Cross Reactivity: Mouse, Rat
• Immunogen: Recombinant iPLA2 (Leu481~Val702 (Accession # P97819)) expressed in E.coli (Immunogen: Recombinant iPLA2 (Leu481~Val702 (Accession # P97819)) expressed in E.coli (MBS2018626)
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 4 degree C for frequent use.; Aliquot and store at -20 degree C for 24 months.; ; Stability Test:; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Mouse Testis lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse iPLA2 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: )

Western Blot (WB)

(Western Blot: Sample: Recombinant iPLA2, Mouse.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin-embedded Kidney tissue))

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Stomach Tissue.))

NCBI and Uniprot Product Information

• NCBI GI #: 312222739
• NCBI GeneID: 53357
• NCBI Accession #: NP_001185952.1
• NCBI GenBank Nucleotide #: NM_001199023.1
• UniProt Accession #: P97819; Q99LA9; Q9JK61
• NCBI Official Full Name: 85/88 kDa calcium-independent phospholipase A2 isoform 2
• NCBI Official Synonym Full Names: phospholipase A2, group VI
• NCBI Official Symbol: Pla2g6
• NCBI Official Synonym Symbols: iPLA2; PNPLA9; BB112799; iPLA2beta; iPLA(2)beta
• NCBI Protein Information: 85/88 kDa calcium-independent phospholipase A2
• UniProt Protein Name: 85/88 kDa calcium-independent phospholipase A2
• UniProt Gene Name: Pla2g6
• UniProt Synonym Gene Names: Pnpla9; CaI-PLA2; GVI PLA2; iPLA2-beta; PNPLA9

Uniprot Description

PLA2G6: Catalyzes the release of fatty acids from phospholipids. It has been implicated in normal phospholipid remodeling, nitric oxide-induced or vasopressin-induced arachidonic acid release and in leukotriene and prostaglandin production. May participate in fas mediated apoptosis and in regulating transmembrane ion flux in glucose-stimulated B-cells. Has a role in cardiolipin (CL) deacylation. Required for both speed and directionality of monocyte MCP1/CCL2-induced chemotaxis through regulation of F- actin polymerization at the pseudopods. Defects in PLA2G6 are the cause of neurodegeneration with brain iron accumulation type 2B (NBIA2B). A neurodegenerative disorder associated with iron accumulation in the brain, primarily in the basal ganglia. It is characterized by progressive extrapyramidal dysfunction leading to rigidity, dystonia, dysarthria and sensorimotor impairment. Defects in PLA2G6 are the cause of neurodegeneration with brain iron accumulation type 2A (NBIA2A); also known as Seitelberger disease. NBIA2A is a neurodegenerative disease characterized by pathologic axonal swelling and spheroid bodies in the central nervous system. Onset is within the first 2 years of life with death by age 10 years. Defects in PLA2G6 are the cause of Parkinson disease type 14 (PARK14). An adult-onset progressive neurodegenerative disorder characterized by parkinsonism, dystonia, severe cognitive decline, cerebral and cerebellar atrophy and absent iron in the basal ganglia on magnetic resonance imaging. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.1.4; Lipid Metabolism - alpha-linolenic acid; Lipid Metabolism - arachidonic acid; Lipid Metabolism - ether lipid; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - linoleic acid; Phospholipase

Chromosomal Location of Human Ortholog: 15|15 E1

Cellular Component: cytoplasm; cytosol; extracellular space; membrane; microtubule organizing center; mitochondrion

Molecular Function: ATP-dependent protein binding; calcium-independent phospholipase A2 activity; calmodulin binding; hydrolase activity; phospholipase A2 activity; protein kinase binding; serine hydrolase activity

Biological Process: antibacterial humoral response; cardiolipin biosynthetic process; chemotaxis; elevation of cytosolic calcium ion concentration; lipid catabolic process; lipid metabolic process; memory; metabolic process; negative regulation of synaptic transmission, glutamatergic; positive regulation of arachidonic acid secretion; positive regulation of blood vessel diameter; positive regulation of ceramide biosynthetic process; positive regulation of exocytosis; positive regulation of protein kinase C signaling; positive regulation of protein phosphorylation; positive regulation of release of cytochrome c from mitochondria; regulation of store-operated calcium channel activity; urinary bladder smooth muscle contraction