Rabbit Protein Kinase C, pan Polyclonal Antibody | anti-Pkcdelta antibody

Protein Kinase C, pan, phosphorylated (PKC)

Cat. Num. AAA623905

• Gene Names: Pkcdelta; CG10524; CG11245; CG42349; DmelCG42349; PKCd
• Reactivity: Human, Monkey, Mouse, Rat
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by Protein A and peptide affinity chromatography.
• Synonyms: Protein Kinase C; pan; Polyclonal Antibody; phosphorylated (PKC); Anti -Protein Kinase C; anti-Pkcdelta antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes endogenous levels of human PKC alpha, beta I, beta II, delta, epsilon and eta isoforms only when phosphorylated at a carboxy-terminal residue homologous to Ser660 of PKC beta II. Does not detect nonphosphorylated PKC or PKC phosphorylated at other sites. Apparent Molecular Weight
• Purity/Purification: Affinity Purified; Purified by Protein A and peptide affinity chromatography.
• Form/Format: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

• Applicable Applications for anti-Pkcdelta antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Dilution: Western Blot: 1:1000; Immunoprecipitation: 1:50; Storage and Stability:; May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser660 of human PKC beta II (KLH).
• Preparation and Storage: 4 degree C (-20 degree C Glycerol)

Western Blot (WB)

(Western blot analysis of Baculovirus expressed PKCbeta and PKCbeta Ser660/Ala mutant, using MBS623905 (upper) or control PKCbeta antibody (lower).)

Western Blot (WB)

(Western blot analysis of extracts from TPA, Go6983 and/or Bisindolylmaleimide treated 293 cells, using MBS623905.)

Western Blot (WB)

(Western blot analysis of Baculovirus expressed PKC isoforms, using MBS623905.)

Related Product Information for anti-Pkcdelta antibody

Activation of PKC is one of the earliest events in the cascade leading to a variety of cellular responses such as secretion, gene expression, proliferation and muscle contraction. PKC isoforms have been classified in three groups: classical PKCs, which are Ca2+ dependent via their C2 domains and are activated by phosphatidylserine (PS), diacylglycerol (DAG) and phorbol esters (TPA or PMA) through their cysteine-rich C1 domains; novel PKCs, which are Ca2+ independent but are still regulated by PS, DAG and TPA; and atypical PKCs, which are Ca2+ independent and do not require PS, DAG or TPA for their activation. These three PKC groups contain a pseudo-substrate or autoinhibitory domain that binds to the substrate binding site in the catalytic domain preventing its activation in the absence of cofactors or activators. Other members have been recently added to the PKC superfamily based on homology within the catalytic domain. PKCmu, or PKD, is regulated by DAG and TPA through its C1 domain. However, PKD is distinguished by a transmembrane domain and PH domain, as well as by its unique substrate recognition and Golgi localization. The PKC-related kinases, or PRKs, lack a C1 domain and thus do not respond to DAG or phorbol esters; instead they can be activated by phosphatidylinositol lipids, and their kinase activity is directly regulated by small GTPases of the Rho family through Rho binding to the homology region 1 (HR1). The activity of PKC is under control by three distinct phosphorylation events. Specifically, threonine 500 at the activation loop, the threonine 641 autophosphorylation site and the serine 660 hydrophobic site at the c-terminus of PKCbeta II are phosphorylated in vivo. The enzyme PDK-1 phosphorylates and activates PKC delta at Thr505 in a phosphoinositide 3 kinase (PI3K) dependent fashion.

Product Categories/Family for anti-Pkcdelta antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 221329868
• NCBI GeneID: 32191
• NCBI Accession #: NP_001138191.1
• NCBI GenBank Nucleotide #: NM_001144719.1
• UniProt Accession #: P83099; Q5BIE9; Q9VYN2
• Molecular Weight: 76,333 Da
• NCBI Official Full Name: protein kinase C delta, isoform D
• NCBI Official Synonym Full Names: Protein kinase C delta
• NCBI Official Symbol: Pkcdelta
• NCBI Official Synonym Symbols: CG10524; CG11245; CG42349; DmelCG42349; PKCd
• NCBI Protein Information: CG42349 gene product from transcript CG42349-RC; CG42349-PB; CG42349-PC; CG42349-PD; Pkcdelta-PB; Pkcdelta-PC; Pkcdelta-PD; protein kinase C; protein kinase C delta
• UniProt Protein Name: Putative protein kinase C delta type homolog
• Protein Family: Putative protein kinase
• UniProt Gene Name: Pkcdelta
• UniProt Entry Name: KPC4_DROME

Uniprot Description

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Sequence similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.Contains 1 AGC-kinase C-terminal domain.Contains 2 phorbol-ester/DAG-type zinc fingers.Contains 1 protein kinase domain.