Rabbit anti-Human Prostate-derived Ets Factor Polyclonal Antibody | anti-SPDEF antibody

Prostate-derived Ets Factor (PDEF)

Cat. Num. AAA611934

• Gene Names: SPDEF; PDEF; bA375E1.3; RP11-375E1__A.3
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified; Purified by epitope affinity chromatography.
• Synonyms: Prostate-derived Ets Factor; Polyclonal Antibody; Prostate-derived Ets Factor (PDEF); Anti -Prostate-derived Ets Factor (PDEF); anti-SPDEF antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Specific for human PDEF. On Western blots, it identifies a single band at ~37.5kD using LNCAP lysates. There is also a weak band at ~55kD which may be due to the glycosylated form of PDEF. Reactivity has been confirmed with human LNCAP prostate carcinoma cell lysates.
• Purity/Purification: Purified; Purified by epitope affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

• Applicable Applications for anti-SPDEF antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 0.1-1ug/ml; Western Blot: 1-3ug/ml
• Immunogen: Synthetic peptide derived from the internal region of human PDEF (prostate-derived Ets factor).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-SPDEF antibody

PDEF (prostate-derived Ets factor) is a 37.5-kD protein that acts as an androgen-independent transcriptional activator for the PSA (prostate specific antigen) promoter. It also interacts directly with the DNA binding domain of androgen receptor and enhances androgen-mediated activation of the PSA promoter. PDEF was shown to have transcript levels 192-fold higher in the peripheral blood of some breast cancer patients in comparison with normal individuals. PDEF mRNA is also

Product Categories/Family for anti-SPDEF antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 530381745
• NCBI GeneID: 25803
• NCBI Accession #: XP_005249045.1
• NCBI GenBank Nucleotide #: XM_005248988.1
• UniProt Accession #: O95238; B4DWH8; F5H778
• Molecular Weight: 37,518 Da
• NCBI Official Full Name: SAM pointed domain-containing Ets transcription factor isoform X1
• NCBI Official Synonym Full Names: SAM pointed domain containing ETS transcription factor
• NCBI Official Symbol: SPDEF
• NCBI Official Synonym Symbols: PDEF; bA375E1.3; RP11-375E1__A.3
• NCBI Protein Information: SAM pointed domain-containing Ets transcription factor; prostate-specific Ets; prostate-derived Ets factor; prostate epithelium-specific Ets transcription factor
• UniProt Protein Name: SAM pointed domain-containing Ets transcription factor
• Protein Family: SAM pointed domain-containing Ets transcription factor
• UniProt Gene Name: SPDEF
• UniProt Synonym Gene Names: PDEF; PSE; Prostate-specific Ets
• UniProt Entry Name: SPDEF_HUMAN

NCBI Description

The protein encoded by this gene belongs to the ETS family of transcription factors. It is highly expressed in the prostate epithelial cells, and functions as an androgen-independent transactivator of prostate-specific antigen (PSA) promoter. Higher expression of this protein has also been reported in brain, breast, lung and ovarian tumors, compared to the corresponding normal tissues, and it shows better tumor-association than other cancer-associated molecules, making it a more suitable target for developing specific cancer therapies. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

SPDEF: May function as an androgen-independent transactivator of the prostate-specific antigen (PSA) promoter. Binds to 5'-GGAT- 3' DNA sequences. May play a role in the regulation of the prostate gland and/or prostate cancer development. Acts as a transcriptional activator for SERPINB5 promoter. Belongs to the ETS family.

Protein type: Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: nucleus

Molecular Function: sequence-specific DNA binding

Biological Process: transcription from RNA polymerase II promoter; positive regulation of cell fate commitment; positive regulation of apoptosis; multicellular organismal development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; cell differentiation; negative regulation of cell fate commitment